Goodier J L, Davidson W S
Department of Biochemistry, Memorial University of Newfoundland, St. John's.
Gene. 1993 Sep 15;131(2):237-42. doi: 10.1016/0378-1119(93)90299-i.
When Atlantic salmon (Salmo salar) genomic DNA is digested with the restriction endonuclease BglI and the fragments separated by agarose-gel electrophoresis, bands corresponding to approximately 430 and 923 bp are visualized after EtdBr staining. The 923-bp band was excised from a preparative gel and used to screen a salmon genomic library for recombinant phage (re-phage) containing the repeat. The BglI repeat element is tandemly arrayed, and an array from one re-phage has been sequenced. The BglI repeats comprise 2.3% of the S. salar genome and have been found in the vicinity of rDNA genes (encoding ribosomal RNA). Southern blot hybridization detects a homologue of the Atlantic salmon BglI repeat in the brown trout (Salmo trutta) genome, but not in other salmonids. However, a DNA fragment with sequence homology to part of the BglI repeat has recently been isolated from Arctic charr (Salvelinus alpinus; S.E. Hartley and W.S.D., unpublished data). In addition, the BglI repeat detects RFLPs in Atlantic salmon.
用限制性内切酶BglI消化大西洋鲑（Salmo salar）基因组DNA，并通过琼脂糖凝胶电泳分离片段，经溴化乙锭（EtdBr）染色后，可观察到对应于约430和923 bp的条带。从制备凝胶中切下923 bp的条带，用于筛选鲑鱼基因组文库中含有该重复序列的重组噬菌体（re - 噬菌体）。BglI重复元件呈串联排列，已对来自一个re - 噬菌体的阵列进行了测序。BglI重复序列占大西洋鲑基因组的2.3%，且已在rDNA基因（编码核糖体RNA）附近发现。Southern印迹杂交在褐鳟（Salmo trutta）基因组中检测到了大西洋鲑BglI重复序列的同源物，但在其他鲑科鱼类中未检测到。然而，最近从北极红点鲑（Salvelinus alpinus；S.E. Hartley和W.S.D.，未发表数据）中分离出了一个与BglI重复序列部分具有序列同源性的DNA片段。此外，BglI重复序列在大西洋鲑中检测到了限制性片段长度多态性（RFLPs）。
