Hess M W, Mittermann I, Luschnig C, Valenta R
Institut für Botanik, Universität Wien, Vienna, Austria.
Histochem Cell Biol. 1995 Dec;104(6):443-51. doi: 10.1007/BF01464334.
Actin was demonstrated for the first time at the EM level in the generative cell of mature angiosperm pollen by using immuno-gold labelling of high-pressure frozen and freeze-substituted Ledebouria socialis Roth anthers. In addition, profilin, an actin-monomer binding protein, is shown to coexist in the generative cell. We attribute the detection of actin and profilin to the applied cryomethods which yield a much better preservation of ultrastructure and antigenicity of delicate cytoskeletal constituents than conventional fixation techniques. Actin labelling was observed within the cytoplasm of the generative cell and became especially clear in close vicinity to microtubular bundles. Filamentous structures congruent with the actin labelling patterns do occur, but are not a frequent feature. Profilin was localised throughout the cytoplasm.
通过对高压冷冻和冷冻置换的紫锦草（Ledebouria socialis Roth）花药进行免疫金标记，首次在成熟被子植物花粉的生殖细胞中在电子显微镜水平上证实了肌动蛋白。此外，肌动蛋白单体结合蛋白——肌动蛋白结合蛋白，也被证明存在于生殖细胞中。我们将肌动蛋白和肌动蛋白结合蛋白的检测归因于所应用的冷冻方法，与传统固定技术相比，该方法能更好地保存精细细胞骨架成分的超微结构和抗原性。在生殖细胞的细胞质中观察到肌动蛋白标记，并且在微管束附近尤其明显。与肌动蛋白标记模式一致的丝状结构确实存在，但并非常见特征。肌动蛋白结合蛋白分布于整个细胞质中。
