[一种研究细胞内游离钙方法的改进]
[Modification of a method for studying free intracellular calcium].
作者信息
Zyma V L, Diachok O M, Hanchurin V V
出版信息
Fiziol Zh (1994). 1995 May-Aug;41(3-4):115-20.
PMID:8777747
Abstract
A microspectrofluorimeter which permits recording excitation and emission spectra of fluorescent dyes (fura-2, indo-1) and measuring Ca(2+)-transients in different cells and smooth muscle strips is described. One-beam and two-beam approaches of recording of spectral characteristic of fluorescent dyes are presented.
摘要
本文描述了一种显微分光荧光计，它能够记录荧光染料（fura-2、indo-1）的激发光谱和发射光谱，并测量不同细胞和平滑肌条中的Ca(2+)瞬变。文中介绍了记录荧光染料光谱特性的单光束和双光束方法。
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