Fernández-Guardiola A, Martínez A, Fernández-Mas R
División de Investigaciones en Neurociencias, Instituto Mexicano de Psiquiatría SSA, México.
Epilepsy Res. 1995 Oct;22(2):127-36. doi: 10.1016/0920-1211(95)00037-2.
The effect of repeated Na-penicillin (PCN) microinjections in the temporal lobe amygdala (AM) of free-moving cats was investigated in order to establish if kindling epileptogenesis is possible with this procedure. The cortical propagation of the PCN-induced post-discharge in AM and the sequence of behavioral changes induced by PCN were similar to those of AM electrical kindling. Nevertheless, the epileptogenic effect of PCN had a different evolution from that of electrical kindling, since some PCN habituation was observed after several doses. Repeated microinjections of PCN did not produce lasting alterations in sleep onset and organization. The only mild changes recorded in the 23 h following PCN microinjections were an increased latency of the first rapid eye movement (REM) sleep episode, a SWS II total time and percentage increase, and, with the highest PCN doses, a not very significant diminution of REM sleep total time. Another finding was the occurrence of REM sleep ponto-geniculo-occipital (PGO) waves, coinciding with a depression of the frequency and amplitude of interictal amygdaloid and cortical spikes. The results showed that a microinjection of PCN in the AM produced a reliable model of interictal spikes, paroxysms and generalized convulsive seizures. Nevertheless, long lasting kindling effect was not observed.
为确定通过该程序是否可能引发点燃式癫痫发生，研究了对自由活动猫的颞叶杏仁核（AM）反复微量注射青霉素钠（PCN）的效果。PCN诱导的AM放电后在皮质的传播以及PCN诱导的行为变化序列与AM电点燃相似。然而，PCN的致癫痫作用与电点燃的发展过程不同，因为在几次给药后观察到了一定程度的PCN习惯化。反复微量注射PCN并未对睡眠开始和睡眠结构产生持久改变。在PCN微量注射后的23小时内记录到的唯一轻微变化是首次快速眼动（REM）睡眠发作的潜伏期延长、慢波睡眠II期总时长和百分比增加，并且在使用最高PCN剂量时，REM睡眠总时长有不太显著的减少。另一个发现是出现了REM睡眠脑桥 - 膝状体 - 枕叶（PGO）波，同时伴有发作间期杏仁核和皮质棘波的频率和幅度降低。结果表明，在AM中微量注射PCN可产生可靠的发作间期棘波、阵发和全身性惊厥发作模型。然而，未观察到持久的点燃效应。
