Ratjen F, Rehn B, Costabel U, Bruch J
Dept of Paediatrics, University of Essen, Germany.
Eur Respir J. 1996 Feb;9(2):328-33. doi: 10.1183/09031936.96.09020328.
Whilst alterations in surfactant components of bronchoalveolar lavage fluid (BALF) have been described in a variety of pulmonary diseases in adults, no information is available on the surfactant composition in children beyond the neonatal period. In order to obtain reference values for the paediatric age group, we have studied phospholipid profiles of BALF in children without pulmonary disease. The study population consisted of 38 children aged 3-15 yrs (mean +/- SD 8 +/- 4 yrs) undergoing elective surgery for nonpulmonary illnesses. Surfactant composition was analysed by high pressure liquid chromatography using both an ultraviolet and a light-scattering detector. Total phospholipid concentration was higher in children under 8 yrs of age and decreased with age. In contrast, the total protein concentration remained constant throughout the age range. No age-related changes in the relative composition of phospholipid fractions could be observed. Surfactant protein A concentrations were highly variable in all age groups. This study provides the first data on surfactant components in bronchoalveolar lavage fluid of healthy children and will be the basis for future studies of children with pulmonary diseases.
虽然在多种成人肺部疾病中已描述了支气管肺泡灌洗液（BALF）表面活性剂成分的改变，但关于新生儿期以外儿童的表面活性剂组成尚无相关信息。为了获得儿科年龄组的参考值，我们研究了无肺部疾病儿童BALF的磷脂谱。研究人群包括38名3至15岁（平均±标准差8±4岁）因非肺部疾病接受择期手术的儿童。使用紫外和光散射检测器通过高压液相色谱法分析表面活性剂组成。8岁以下儿童的总磷脂浓度较高，并随年龄增长而降低。相比之下，总蛋白浓度在整个年龄范围内保持恒定。未观察到磷脂组分相对组成的年龄相关变化。表面活性剂蛋白A浓度在所有年龄组中变化很大。本研究提供了健康儿童支气管肺泡灌洗液中表面活性剂成分的首批数据，将作为未来对患有肺部疾病儿童研究的基础。
