Takemura T, Okada M, Akano N, Murakami K, Hino S, Yagi K, Takekoshi Y, Yoshioka K
Department of Pediatrics, Kinki University School of Medicine, Osaka, Japan.
J Pathol. 1996 Mar;178(3):343-51. doi: 10.1002/(SICI)1096-9896(199603)178:3<343::AID-PATH481>3.0.CO;2-H.
The expression of the protein products and mRNA of c-fos, c-myc, p53, and c-raf was examined in normal renal tissues and biopsy specimens from 73 patients with various glomerular diseases. Immunofluorescent staining showed that there were cell nuclei stained for c-Fos, c-Myc, and p53, and cytoplasm positive for c-Raf, in the glomeruli of patients with proliferative types of glomerulonephritis, including IgA nephritis and lupus nephritis, and in patients with focal glomerular sclerosis. Glomerular expression of c-fos and c-myc mRNA was detected by in situ hybridization. The number of proto-oncogene-positive glomerular cells was significantly higher in lupus nephritis, IgA nephritis, and focal segmental sclerosis, as compared with minimal change nephrotic syndrome and normal specimens. In IgA nephritis, the population of glomerular cells positive for c-Fos and c-Myc and the grade of c-Raf immunoreactivity were significantly correlated with the proportion of proliferating cell nuclear antigen (PCNA)-positive glomerular cells, with histological grading of mesangial hypercellularity and matrix increase, and with the magnitude of proteinuria. These data indicate that proto-oncogene expression is associated with mesangial proliferation and matrix expansion in proliferative types of glomerulonephritis and in focal glomerular sclerosis.
在73例患有各种肾小球疾病的患者的正常肾组织和活检标本中，检测了c-fos、c-myc、p53和c-raf的蛋白质产物及mRNA的表达。免疫荧光染色显示，在增生性肾小球肾炎患者（包括IgA肾病和狼疮性肾炎）以及局灶性肾小球硬化患者的肾小球中，存在c-Fos、c-Myc和p53染色的细胞核，c-Raf染色呈阳性的细胞质。通过原位杂交检测c-fos和c-myc mRNA的肾小球表达。与微小病变肾病综合征和正常标本相比，狼疮性肾炎、IgA肾病和局灶节段性硬化中原癌基因阳性的肾小球细胞数量显著更高。在IgA肾病中，c-Fos和c-Myc阳性的肾小球细胞数量以及c-Raf免疫反应性分级与增殖细胞核抗原（PCNA）阳性的肾小球细胞比例、系膜细胞增多和基质增加的组织学分级以及蛋白尿程度显著相关。这些数据表明，原癌基因表达与增生性肾小球肾炎和局灶性肾小球硬化中的系膜增殖和基质扩张有关。
