Nakopoulou L, Stefanaki K, Salpigidis K, Boletis J, Papadakis J, Zeiss P M, Vosnides G
Department of Pathology, Medical School, University of Athens, Greece.
Histol Histopathol. 1997 Jul;12(3):655-62.
Proliferating cell nuclear antigen (PCNA)/cyclin is an acidic nuclear protein increasing from the late G1 to S phases of the cell cycle and whose detection parallels other standard methods for assessing cell proliferation. The aim of this study was to investigate PCNA expression in normal and diseased human kidneys, in order to clarify cell proliferation in renal tissue and to define a possible correlation of its expression with various types of glomerulonephritis (GN). The immunohistochemical avidin-biotin complex (ABC) method was used for the demonstration of PCNA applying the monoclonal antibody PC-10 to paraffin sections from: 10 normal kidneys, 55 renal biopsies with various types of proliferative GN (PGN), 44 renal biopsies with various types of non proliferative GN (NPGN). In PCNA-positive renal biopsies with GN the antigen showed a heterogenous nuclear expression in occasional or few mesangial and glomerular epithelial cells as well as in a greater number of tubular epithelial cells. PCNA was expressed in 20% of normal kidneys and in 38% of renal biopsies with GN. The frequency of PCNA expression was significantly increased in the cases of PGN (47%) compared to that observed in the cases of NPGN (27%) (p = 0.03). PCNA was detected in 10/24 cases of IgA nephropathy, in 3/4 cases of IgM nephropathy, in 5/14 of other types of primary PGN and in 8/13 of secondary PGN. PCNA expression was not correlated with the degree of mesangial cellularity in PGN. Moreover, there was no significant difference in PCNA expression between primary and secondary PGN. PCNA demonstrated an intense expression in the majority of epithelial cells forming cellular crescents in 8/11 cases of PGN. In conclusion, PCNA was observed more frequently in diseased than in normal kidneys. The significant increase in the frequency of PCNA intraglomerular expression in PGN suggests that PCNA has a certain value in the assessment of mesangial proliferation. Moreover, the increased PCNA expression in tubular epithelial cells especially in PGN, indicates their proliferative state and may be correlated with their proposed activation and role in the progression of renal injury.
增殖细胞核抗原（PCNA）/细胞周期蛋白是一种酸性核蛋白，在细胞周期的G1晚期至S期含量增加，其检测结果与评估细胞增殖的其他标准方法相似。本研究旨在调查PCNA在正常和患病人类肾脏中的表达情况，以阐明肾组织中的细胞增殖，并确定其表达与各种类型肾小球肾炎（GN）之间可能存在的相关性。采用免疫组织化学抗生物素蛋白-生物素复合物（ABC）法，将单克隆抗体PC-10应用于以下石蜡切片来检测PCNA：10个正常肾脏、55例各种类型增殖性GN（PGN）的肾活检标本、44例各种类型非增殖性GN（NPGN）的肾活检标本。在患有GN的PCNA阳性肾活检标本中，抗原在偶尔或少数系膜细胞和肾小球上皮细胞以及大量肾小管上皮细胞中呈异质性核表达。PCNA在20%的正常肾脏和38%的患有GN的肾活检标本中表达。与NPGN病例（27%）相比，PGN病例（47%）中PCNA表达频率显著增加（p = 0.03）。在24例IgA肾病中有10例检测到PCNA，在4例IgM肾病中有3例，在其他类型的原发性PGN的14例中有5例，在继发性PGN的13例中有8例。PCNA表达与PGN中系膜细胞增多程度无关。此外，原发性和继发性PGN之间的PCNA表达无显著差异。在11例PGN中有8例，PCNA在形成细胞性新月体的大多数上皮细胞中呈强表达。总之，在患病肾脏中比在正常肾脏中更频繁地观察到PCNA。PGN中肾小球内PCNA表达频率的显著增加表明PCNA在评估系膜增生方面具有一定价值。此外，肾小管上皮细胞中PCNA表达增加，尤其是在PGN中，表明它们的增殖状态，可能与其在肾损伤进展中所提出的激活作用和作用相关。
