与DNA复合的大肠杆菌复制终止蛋白的结晶及初步X射线分析。

Crystallization and preliminary X-ray analysis of the Escherichia coli replication terminator protein complexed with DNA.

作者信息

Kamada K, Ohsumi K, Horiuchi T, Shimamoto N, Morikawa K

机构信息

Department of Genetics, Graduate University for Advanced Studies, Shizuoka, Japan.

出版信息

Proteins. 1996 Mar;24(3):402-3. doi: 10.1002/(SICI)1097-0134(199603)24:3<402::AID-PROT14>3.0.CO;2-Q.

DOI:10.1002/(SICI)1097-0134(199603)24:3<402::AID-PROT14>3.0.CO;2-Q

PMID:8778788

Abstract

Crystals of the Escherichia coli replication terminator protein (Tus) complexed with its binding site DNA were obtained by a microdialysis method using PEG 4000. They belong to the tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 with the unit cell parameter: a = 68.1 A, c = 230.7 A and contain one protein-DNA complex in an asymmetric unit. The native data set has been collected to 2.7 A resolution.

摘要

通过使用聚乙二醇4000的微透析方法获得了与结合位点DNA复合的大肠杆菌复制终止蛋白（Tus）的晶体。它们属于四方晶系空间群P4(1)2(1)2或P4(3)2(1)2，晶胞参数为：a = 68.1 Å，c = 230.7 Å，在不对称单元中包含一个蛋白质-DNA复合物。已收集到分辨率为2.7 Å的天然数据集。