Nieto M J, Cañizo C, Corral M, Almeida J, San Miguel J
Hospital Universitario, Salamanca, España.
Sangre (Barc). 1996 Feb;41(1):29-35.
To analyze the effect of conventional chemotherapy on haematopoietic progenitor cells (HPC) from peripheral blood (PB).
The present work studies the behaviour of CFU-GM from PB in 47 patients subjected to conventional chemotherapy with the following diagnoses: non-Hodgkin's lymphoma (NHL): 16; Hodgkin's disease (HD): 8; multiple myeloma (MM): 5; acute leukaemia (AL): 8; solid tumor (ST): 10. A total of 57 studies was performed. As controls, 51 samples of PB from healthy subjects were used. Cultures were made in semisolid medium (methylcellulose), using PHA-LCM as stimulant, evaluating the growth of clusters (cl) and colonies (col) on days 7 and 14.
Our findings show that in the overall series of patients the number of clusters from days 7 and 14 and of colonies-7--that is the most differentiated progenitor cells--were decreased with respect to the controls while colonies-14 were slightly increased. When the patients were separated by diagnostic groups, the col-14 were found to be increased in ST (p = 0.03) and in AL; in NHL col-14 were the same as in the controls. In the patients with HD all progenitor cells, including col-14 were much lower than the control values (p = 0.005). In order to determine whether the kind or the amount of chemotherapy received might affect these progenitor cells, we have analyzed the cases of NHL, 11 patients treated with moderate chemotherapy versus 12 patients receiving intensive chemotherapy: a stronger degree of growth in the latter, although with no significant differences. On comparing the cases that had received little previous chemotherapy (< 5 courses) against those who had received more than 5 courses growth was seen to be stronger in the patients who had received the fewest courses (p = 0.04).
These results indicate that the HPC from PB are scanty in certain diseases, such as HD and that in all lymphomas the collection of these cells for transplantation purposes should be performed before they have damaged by an excessive number of chemotherapy courses.
分析传统化疗对外周血造血祖细胞（HPC）的影响。
本研究观察了47例接受传统化疗患者外周血中粒-巨噬细胞集落形成单位（CFU-GM）的行为，这些患者的诊断如下：非霍奇金淋巴瘤（NHL）：16例；霍奇金病（HD）：8例；多发性骨髓瘤（MM）：5例；急性白血病（AL）：8例；实体瘤（ST）：10例。共进行了57项研究。作为对照，使用了51份健康受试者的外周血样本。在半固体培养基（甲基纤维素）中进行培养，使用PHA-LCM作为刺激物，在第7天和第14天评估集落（cl）和集落（col）的生长情况。
我们的研究结果表明，在整个患者系列中，与对照组相比，第7天和第14天的集落数量以及最分化的祖细胞集落-7的数量减少，而集落-14略有增加。当按诊断组对患者进行分类时，发现实体瘤（p = 0.03）和急性白血病患者的集落-14增加；非霍奇金淋巴瘤患者的集落-14与对照组相同。霍奇金病患者的所有祖细胞，包括集落-14，均远低于对照值（p = 0.005）。为了确定所接受化疗的种类或剂量是否会影响这些祖细胞，我们分析了非霍奇金淋巴瘤的病例，11例接受中度化疗的患者与12例接受强化化疗的患者：后者的生长程度更强，尽管无显著差异。将先前接受化疗较少（< 5个疗程）的病例与接受超过5个疗程的病例进行比较，发现接受疗程最少的患者生长更强（p = 0.04）。
这些结果表明，某些疾病（如霍奇金病）外周血中的造血祖细胞数量较少，并且在所有淋巴瘤中，为移植目的采集这些细胞应在它们因过多化疗疗程而受损之前进行。
