用于结核诊断的IS6110聚合酶链反应与Amplicor的比较。
Comparison of polymerase chain reaction for IS6110 and Amplicor in the diagnosis of tuberculosis.
作者信息
Dilworth J P, Goyal M, Young D B, Shaw R J
机构信息
Department of Respiratory Medicine, St Mary's Hospital Medical School, London, UK.
出版信息
Thorax. 1996 Mar;51(3):320-2. doi: 10.1136/thx.51.3.320.
BACKGROUND
Polymerase chain reaction (PCR) amplification of Mycobacterium tuberculosis DNA offers the potential of a sensitive and specific diagnostic test for tuberculosis. To evaluate this technique from the clinician's perspective, samples were collected from patients with chronic respiratory disease and the sensitivity and specificity of a newly introduced commercially available PCR kit (Amplicor) was compared with that of an established method to detect the target sequence IS6110.
METHODS
Sputum or bronchial washings from patients with active tuberculosis, previously treated tuberculosis or other selected respiratory illnesses were analysed by both techniques and their sensitivity and specificity determined.
RESULTS
Amplicor was more specific than IS6110 in the diagnosis of active infection (98% versus 79%). Both techniques were equally sensitive (92%).
CONCLUSION
These results suggest that analysis of respiratory samples by Amplicor PCR in inner city populations of patients has greater specificity for a diagnosis of active tuberculosis than PCR for IS6110, and thus Amplicor PCR may aid the clinician in making a diagnosis of active tuberculosis.
背景
结核分枝杆菌DNA的聚合酶链反应（PCR）扩增为结核病提供了一种灵敏且特异的诊断检测方法。为了从临床医生的角度评估该技术，我们从患有慢性呼吸道疾病的患者中采集样本，将一种新推出的市售PCR试剂盒（Amplicor）与一种已确立的检测靶序列IS6110的方法的敏感性和特异性进行了比较。
方法
采用这两种技术对活动性结核病患者、既往接受过治疗的结核病患者或其他选定呼吸道疾病患者的痰液或支气管灌洗液进行分析，并确定其敏感性和特异性。
结果
在活动性感染的诊断中，Amplicor比IS6110更具特异性（分别为98%和79%）。两种技术的敏感性相同（均为92%）。
结论
这些结果表明，在城市中心患者群体中，使用Amplicor PCR分析呼吸道样本对活动性结核病的诊断比检测IS6110的PCR具有更高的特异性，因此Amplicor PCR可能有助于临床医生诊断活动性结核病。
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