从红酵母鲑色掷孢酵母中克隆醛还原酶基因，并对该基因及其产物进行表征。
Cloning of the aldehyde reductase gene from a red yeast, Sporobolomyces salmonicolor, and characterization of the gene and its product.
作者信息
Kita K, Matsuzaki K, Hashimoto T, Yanase H, Kato N, Chung M C, Kataoka M, Shimizu S
机构信息
Department of Biotechnology, Tottori University, Japan. kita @bio.tottori-u.ac.jp
出版信息
Appl Environ Microbiol. 1996 Jul;62(7):2303-10. doi: 10.1128/aem.62.7.2303-2310.1996.
Abstract
An NADPH-dependent aldehyde reductase (ALR) isolated from a red yeast, Sporobolomyces salmonicolor, catalyzes the reduction of a variety of carbonyl compounds. To investigate its primary structure, we cloned and sequenced the cDNA coding for ALR. The aldehyde reductase gene (ALR) comprises 969 bp and encodes a polypeptide of 35,232 Da. The deduced amino acid sequence showed a high degree of similarity to other members of the aldo-keto reductase superfamily. Analysis of the genomic DNA sequence indicated that the ALR gene was interrupted by six introns (two in the 5' noncoding region and four in the coding region). Southern hybridization analysis of the genomic DNA from S. salmonicolor indicated that there was one copy of the gene. The ALR gene was expressed in Escherichia coli under the control of the tac promoter. The enzyme expressed in E. coli was purified to homogeneity and showed the same catalytic properties as did the enzyme from S. salmonicolor.
摘要
从红酵母鲑色掷孢酵母中分离出的一种依赖烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的醛还原酶（ALR）可催化多种羰基化合物的还原反应。为研究其一级结构，我们克隆并测序了编码ALR的cDNA。醛还原酶基因（ALR）由969个碱基对组成，编码一个分子量为35232道尔顿的多肽。推导的氨基酸序列与醛酮还原酶超家族的其他成员高度相似。基因组DNA序列分析表明，ALR基因被6个内含子打断（5'非编码区有2个，编码区有4个）。对鲑色掷孢酵母基因组DNA的Southern杂交分析表明该基因有一个拷贝。ALR基因在tac启动子的控制下在大肠杆菌中表达。在大肠杆菌中表达的酶被纯化至同质，并且表现出与来自鲑色掷孢酵母的酶相同的催化特性。
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1
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Appl Environ Microbiol. 1988 Jul;54(7):1770-5. doi: 10.1128/aem.54.7.1770-1775.1988.
2
Probing the active site of human aldose reductase. Site-directed mutagenesis of Asp-43, Tyr-48, Lys-77, and His-110.探究人类醛糖还原酶的活性位点。天冬氨酸-43、酪氨酸-48、赖氨酸-77和组氨酸-110的定点诱变。
J Biol Chem. 1993 Dec 5;268(34):25687-93.
3
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Biochemistry. 1994 Mar 1;33(8):2021-32. doi: 10.1021/bi00174a007.
4
Purification and characterization of human-brain aldose reductase.人脑中醛糖还原酶的纯化与特性分析
Eur J Biochem. 1982 Oct;127(2):279-84. doi: 10.1111/j.1432-1033.1982.tb06867.x.
5
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Proc Natl Acad Sci U S A. 1984 Nov;81(22):6929-33. doi: 10.1073/pnas.81.22.6929.
6
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Gene. 1982 Oct;19(3):269-76. doi: 10.1016/0378-1119(82)90016-6.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.大肠杆菌16S核糖体RNA的3'末端序列：与无义三联体及核糖体结合位点的互补性
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9
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10
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