Bogan B W, Schoenike B, Lamar R T, Cullen D
Department of Cell and Molecular Biology, University of Wisconsin-Madison 53706, USA.
Appl Environ Microbiol. 1996 Jul;62(7):2381-6. doi: 10.1128/aem.62.7.2381-2386.1996.
mRNA extraction from soil and quantitation by competitive reverse transcription-PCR were combined to study the expression of three manganese peroxidase (MnP) genes during removal of polycyclic aromatic hydrocarbons from cultures of Phanerochaete chrysosporium grown in presterilized soil. Periods of high mnp transcript levels and extractable MnP enzyme activity were temporally correlated, although separated by a short (1- to 2-day) lag period. This time frame also coincided with maximal rates of fluorene oxidation and chrysene disappearance in soil cultures, supporting the hypothesis that high ionization potential polycyclic aromatic hydrocarbons are oxidized in soil via MnP-dependent mechanisms. The patterns of transcript abundance over time in soil-grown P. chrysosporium were similar for all three of the mnp mRNAs studied, indicating that transcription of this gene family may be coordinately regulated under these growth conditions.
将从土壤中提取mRNA并通过竞争性逆转录聚合酶链反应进行定量相结合，以研究在从预灭菌土壤中生长的黄孢原毛平革菌培养物中去除多环芳烃期间三种锰过氧化物酶（MnP）基因的表达。尽管存在短暂（1至2天）的滞后阶段，但mnp转录本高水平期与可提取的MnP酶活性在时间上相关。这个时间框架也与土壤培养物中芴氧化和屈消失的最大速率相吻合，支持了高电离势多环芳烃在土壤中通过依赖MnP的机制被氧化的假设。在所研究的所有三种mnp mRNA中，土壤生长的黄孢原毛平革菌中随时间变化的转录本丰度模式相似，表明该基因家族的转录在这些生长条件下可能受到协调调节。
