Bello R, Cardillo E, Martínez R
Instituto de Ciencia y Tecnología de Alimentos, Facultad de Ciencias, Universidad Central de Venezuela.
Arch Latinoam Nutr. 1993 Sep;43(3):221-7.
Microbial fish silage was produced from a mixture of several fish species that belong to the shrimp by-catch. They were mixed with molasses, fruits (pineapple and papaya), sorbate and a starter of Lactobacillus plantarum ATCC8014. Process was evaluated by pH, acidity, consistency, exudate liquid, non-protein nitrogen, total volatile bases, microbial and toxicological tests. Results indicated that acid production and pH reduction occurs during the first two days of processing, later these values were maintained stable during 64 storage days. Total volatile bases increased during storage period. Consistency, non-protein nitrogen and exudate liquid showed that hydrolysis and liquefaction occurs during the first 8 days of processing. Raw material showed high counts of aerobic mesophilic and psicrotrophic organisms, in addition to Pseudomonas, coliform and S. aureus. However silage showed only a few aerobic mesophilic organisms due to low pH values and development of lactic acid bacteria. Silage dehydration reduces possibilities of microbial growth, and only spores of Bacillus were observed. Low levels of lead, mercury and chrome were detected in the dry silage. Proximal analysis values did not change during process and storage period.
微生物鱼青贮饲料由属于虾类副渔获物的几种鱼类混合制成。它们与糖蜜、水果（菠萝和木瓜）、山梨酸盐以及植物乳杆菌ATCC8014发酵剂混合。通过pH值、酸度、稠度、渗出液、非蛋白氮、总挥发性碱、微生物和毒理学测试对该过程进行评估。结果表明，在加工的前两天会产生酸并降低pH值，之后在64天的储存期内这些值保持稳定。储存期间总挥发性碱增加。稠度、非蛋白氮和渗出液表明在加工的前8天会发生水解和液化。原材料显示需氧嗜温菌和嗜冷菌数量较多，此外还有假单胞菌、大肠菌群和金黄色葡萄球菌。然而，由于pH值较低且乳酸菌生长，青贮饲料中仅显示出少量需氧嗜温菌。青贮饲料脱水降低了微生物生长的可能性，仅观察到芽孢杆菌的孢子。在干青贮饲料中检测到低水平的铅、汞和铬。在加工和储存期间，近似分析值没有变化。
