Samour J H, Kaaden O R, Wernery U, Bailey T A
Veterinary Science Department, National Avian Research Centre, Abu Dhabi, United Arab Emirates.
Zentralbl Veterinarmed B. 1996 Jul;43(5):287-92. doi: 10.1111/j.1439-0450.1996.tb00316.x.
An epornitic of avian pox occurred in a flock of 123 houbara bustards (Chlamydotis undulata macqueenii) received at the Sulman Falcon Hospital in the State of Bahrain in February 1993. Birds displayed conjunctivitis, excessive lacrimation and papilloma-like growths forming amorphous clusters on the third eyelid and on the conjunctiva. Examination of eyelid samples under transmission electron microscopy revealed pox virus particles displaying the classical morphology of vaccinia-avipox virions. Typical pox lesions were also detected 5 days post infection (p.i.) on chorioallantoic membrane (CAM). The virus titre on CAM was 10(7) focus-forming units (FFU)/ml. In tissue culture, only a slight cytopathogenic effect (CPE) was detected 5 days p.i. the virus titre on cell cultures was 10(4.5)FFU/ml. The virus infection in cell culture appeared to be abortive and no CPE was seen after three passages in secondary chicken embryo fibroblasts. No neutralization of the cell-grown virus was detected on serological studies using antisera directed against fowl, pigeon, canary and sparrowpox viruses. The discussion is framed around the different cultural properties of the houbara bustard pox virus isolate and its relationship to other known viral strains.
1993年2月，巴林国苏尔曼猎鹰医院接收了一群123只鸨（Chlamydotis undulata macqueenii），其中发生了禽痘流行。鸟类表现出结膜炎、流泪过多以及乳头状瘤样生长，在第三眼睑和结膜上形成无定形簇。在透射电子显微镜下检查眼睑样本，发现痘病毒颗粒呈现出痘苗-禽痘病毒粒子的典型形态。感染后5天（p.i.），在绒毛尿囊膜（CAM）上也检测到典型的痘病变。CAM上的病毒滴度为10(7)个焦点形成单位（FFU）/毫升。在组织培养中，感染后5天仅检测到轻微的细胞病变效应（CPE），细胞培养物上的病毒滴度为10(4.5)FFU/毫升。细胞培养中的病毒感染似乎是流产性的，在二代鸡胚成纤维细胞中传代三次后未观察到CPE。使用针对禽、鸽、金丝雀和麻雀痘病毒的抗血清进行血清学研究，未检测到细胞培养生长病毒的中和作用。讨论围绕鸨痘病毒分离株的不同培养特性及其与其他已知病毒株的关系展开。
