Smith P M, Ferguson A V
Department of Physiology, Queen's University, Kingston, Ontario, Canada.
Am J Physiol. 1996 Feb;270(2 Pt 2):R342-47. doi: 10.1152/ajpregu.1996.270.2.R342.
Extracellular single-unit recordings were obtained from area postrema neurons (AP), and peristimulus histograms were used to determine the effects of paraventricular nucleus (PVN) stimulation on these cells from anesthetized Sprague-Dawley rats. Of 91 AP cells tested, 30.8% responded to PVN stimulation with a short-latency (28.2 +/- 3.3 ms, mean +/- SE), short-duration (49.3 +/- 8.0 ms) excitation, whereas 8.6% were inhibited. In animals that had stimulation sites outside of PVN (non-PVN), only 4 of the 72 AP cells tested (5.6%) were influenced by stimulation. These excitatory effects of PVN stimulation on AP neurons were unaffected by V1-receptor blockade. Of 93 nucleus of the solitary tract (NTS) cells tested, 38.9% responded to PVN stimulation with a short-latency (18.5 +/- 2.4 ms), short-duration (48.8 +/- 9.6 ms) excitation and 22.2% with short-latency (20.75 +/- 4.1 ms), long-duration (204.4 +/- 44.9 ms) inhibitions. In contrast, non-PVN stimulation sites influenced only 19% of NTS neurons tested, all of which were excited. These data demonstrate that activation of PVN neurons elicits excitatory effects on the majority of AP neurons influenced. They further emphasize the potential significance of descending hypothalamic inputs in controlling neuronal activity in this circumventricular organ.
从最后区神经元（AP）获取细胞外单单位记录，并使用刺激后直方图来确定来自麻醉的Sprague-Dawley大鼠的室旁核（PVN）刺激对这些细胞的影响。在测试的91个AP细胞中，30.8%对PVN刺激有短潜伏期（28.2±3.3毫秒，平均值±标准误）、短持续时间（49.3±8.0毫秒）的兴奋反应，而8.6%受到抑制。在刺激部位位于PVN之外（非PVN）的动物中，测试的72个AP细胞中只有4个（5.6%）受到刺激影响。PVN刺激对AP神经元的这些兴奋作用不受V1受体阻断的影响。在测试的93个孤束核（NTS）细胞中，38.9%对PVN刺激有短潜伏期（18.5±2.4毫秒）、短持续时间（48.8±9.6毫秒）的兴奋反应，22.2%有短潜伏期（20.75±4.1毫秒）、长持续时间（204.4±44.9毫秒）的抑制反应。相比之下，非PVN刺激部位仅影响了19%的测试NTS神经元，所有这些神经元均被兴奋。这些数据表明，PVN神经元的激活对受影响的大多数AP神经元产生兴奋作用。它们进一步强调了下丘脑下行输入在控制这个室周器官神经元活动中的潜在重要性。
