Gödecke S, Decking U K, Gödecke A, Schrader J
Institut für Herz- und Kreislaufphysiologie, Heinrich-Heine-Universität, Dusseldorf, Germany.
Am J Physiol. 1996 Feb;270(2 Pt 1):C570-7. doi: 10.1152/ajpcell.1996.270.2.C570.
We cloned and sequenced the cDNA as well as the genomic DNA of the P2u receptor gene from the rat. The coding region of the gene is not interrupted by introns. P2u is expressed in a variety of rat organs with pronounced differences of expression intensities. Highest expression was found in liver and testis, while no expression could be detected in the brain. High P2u expression was found in primary microvascular endothelial cells from the rat heart, but not in cardiac myocytes. By in situ analysis, we localized P2u expression in epithelial cells of esophagus and bronchi. Functional analysis revealed that, in isolated perfused rat hearts, the P2u ligands UTP and ATP induce a pronounced vasodilation of coronary blood vessels. In contrast, UMP and uridine, the degradative products of UTP, act as potent vasoconstrictors. Our experiments suggest that, in the rat heart, endothelial P2u receptors are involved in the ATP/UTP-mediated vasodilation of coronary blood vessels.
我们克隆并测序了大鼠P2u受体基因的cDNA以及基因组DNA。该基因的编码区没有被内含子打断。P2u在大鼠的多种器官中表达，表达强度存在明显差异。在肝脏和睾丸中表达最高，而在脑中未检测到表达。在大鼠心脏的原代微血管内皮细胞中发现高P2u表达，但在心肌细胞中未发现。通过原位分析，我们将P2u表达定位在食管和支气管的上皮细胞中。功能分析表明，在离体灌注的大鼠心脏中，P2u配体UTP和ATP可诱导冠状动脉明显的血管舒张。相反，UTP的降解产物UMP和尿苷则作为强效血管收缩剂。我们的实验表明，在大鼠心脏中，内皮P2u受体参与了ATP/UTP介导的冠状动脉血管舒张。
