Supino R, Casazza A M, Di Marco A
Tumori. 1977 Jan-Feb;63(1):31-42. doi: 10.1177/030089167706300105.
This paper reports the effects of daunorubicin and adriamycin on DNA and RNA synthesis of in vitro cultured mouse embryo fibroblasts (MEF) stimulated by fetal calf serum (FCS). The addition of FCS to quiescent MEF cultures brings about a wave of RNA synthesis, followed by DNA synthesis which starts between 8 and 12 h after change of medium and proceed for up to 24 h. These cells are therefore partially synchronized. The level of DNA synthesis depends on the amount of FCS added. Daunorubicin and adriamycin are almost equally effective in inhibiting DNA synthesis, as well as cell proliferation, which takes place later. Adriamycin is more active than daunorubicin on RNA synthesis. In cultures treated for an 8 h period starting at different times after FCS addition, the highest DNA synthesis inhibition is achieved by treatment during the first 8 h, when DNA synthesis has not yet started. The cellular uptake of daunorubicin is constantly higher than that of adriamycin, in any experimental condition tested. The results show that FCS-stimulated MEFcan provide a simple method for studying the effects of anthracycline antibiotics on partially synchronized cells.
本文报道了柔红霉素和阿霉素对胎牛血清（FCS）刺激的体外培养小鼠胚胎成纤维细胞（MEF）DNA和RNA合成的影响。向静止的MEF培养物中添加FCS会引发一波RNA合成，随后是DNA合成，DNA合成在更换培养基后8至12小时开始，并持续长达24小时。因此，这些细胞处于部分同步状态。DNA合成水平取决于添加的FCS量。柔红霉素和阿霉素在抑制DNA合成以及随后发生的细胞增殖方面几乎同样有效。阿霉素在RNA合成方面比柔红霉素更具活性。在添加FCS后不同时间开始处理8小时的培养物中，在DNA合成尚未开始的前8小时进行处理可实现最高的DNA合成抑制。在任何测试的实验条件下，柔红霉素的细胞摄取量始终高于阿霉素。结果表明，FCS刺激的MEF可为研究蒽环类抗生素对部分同步细胞的影响提供一种简单方法。
