Zheng D, Wooter M H, Zhou Q, Dohm G L
Department of Biochemistry, East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
Biochem Biophys Res Commun. 1996 Aug 23;225(3):747-50. doi: 10.1006/bbrc.1996.1245.
Expression of the ob gene is subject to nutritional as well as hormonal regulation to control fat storage. In the present study we investigated the effect of acute exercise and long-term exercise training on ob mRNA levels in rat adipose tissue. Northern blot analysis showed that a single bout of exercise significantly decreased ob mRNA levels approximately 30% immediately and 3 hr after exercise. After 4 weeks of exercise training the ob mRNA level was significantly decreased approximately 48% at 2 hr after the last training session compared to the control group. These data demonstrated that exercise has a transient effect on ob gene expression and suggest that regulation of ob gene expression may help control energy balance following exercise.
ob基因的表达受营养和激素调节以控制脂肪储存。在本研究中，我们调查了急性运动和长期运动训练对大鼠脂肪组织中ob mRNA水平的影响。Northern印迹分析表明，单次运动在运动后即刻和3小时可使ob mRNA水平显著降低约30%。运动训练4周后，与对照组相比，末次训练后2小时ob mRNA水平显著降低约48%。这些数据表明运动对ob基因表达有短暂影响，并提示ob基因表达的调节可能有助于运动后控制能量平衡。
