Zheng D, Jones J P, Usala S J, Dohm G L
Department of Biochemistry, East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
Biochem Biophys Res Commun. 1996 Jan 17;218(2):434-7. doi: 10.1006/bbrc.1996.0077.
The recently cloned obese (ob) gene encodes a fat-specific mRNA and a protein which is believed to regulate satiety and metabolic rate so as to control fat storage. In the present study we investigated rat ob mRNA levels in different fat depots and the effects of insulin infusion on ob mRNA expression. Northern blot analysis showed the abdominal fat (epididymal and perirenal fat pads) had higher ob mRNA levels than subcutaneous fat. After a 2.5-hr infusion of insulin into fasted rats, ob mRNA levels were increased by 3.0 and 1.9-fold in epididymal and perirenal fat pads, respectively. However, no change of ob mRNA level was observed in the subcutaneous fat depot. These data demonstrate adipose depot site-specific expression of ob mRNA and support the hypothesis that insulin plays an important role in the expression of ob mRNA.
最近克隆出的肥胖（ob）基因编码一种脂肪特异性mRNA和一种蛋白质，据信该蛋白质可调节饱腹感和代谢率，从而控制脂肪储存。在本研究中，我们调查了大鼠不同脂肪库中ob mRNA的水平以及胰岛素输注对ob mRNA表达的影响。Northern印迹分析显示，腹部脂肪（附睾和肾周脂肪垫）的ob mRNA水平高于皮下脂肪。在禁食大鼠中输注胰岛素2.5小时后，附睾和肾周脂肪垫中的ob mRNA水平分别增加了3.0倍和1.9倍。然而，皮下脂肪库中未观察到ob mRNA水平的变化。这些数据证明了ob mRNA在脂肪库部位的特异性表达，并支持胰岛素在ob mRNA表达中起重要作用这一假说。
