Miquel K, Pradines A, Favre G
Laboratoire d'Oncologie Cellulaire et Moléculaire, INSERM U397, Toulouse, France.
Biochem Biophys Res Commun. 1996 Aug 23;225(3):869-76. doi: 10.1006/bbrc.1996.1265.
The effects of exogenous isoprenoids were investigated on A549 human lung adenocarcinoma cells. Among the tested isoprenoids, only farnesol and geranylgeraniol induce actin cytoskeleton disorganization, growth inhibition, and apoptosis. In contrast, desmosterol leads only to growth inhibition. We show that all tested isoprenoids are potent inhibitors of HMG CoA reductase activity, the sterols being the most powerful while they induce neither F-actin disorganization nor apoptosis. Thus the molecular mechanisms induced by farnesol and geranylgeraniol appear independent of reductase regulation. Our results point out the specific role of farnesol and geranylgeraniol on actin cytoskeleton organization and apoptosis in adenocarcinoma cells.
研究了外源性类异戊二烯对A549人肺腺癌细胞的影响。在所测试的类异戊二烯中，只有法尼醇和香叶基香叶醇可诱导肌动蛋白细胞骨架紊乱、生长抑制和细胞凋亡。相比之下，去氢胆固醇仅导致生长抑制。我们发现，所有测试的类异戊二烯都是HMG CoA还原酶活性的有效抑制剂，甾醇是最有效的，同时它们既不诱导F-肌动蛋白紊乱也不诱导细胞凋亡。因此，法尼醇和香叶基香叶醇诱导的分子机制似乎与还原酶调节无关。我们的结果指出了法尼醇和香叶基香叶醇在腺癌细胞中对肌动蛋白细胞骨架组织和细胞凋亡的特定作用。
