Miwa W, Yasuda J, Murakami Y, Yashima K, Sugano K, Sekine T, Kono A, Egawa S, Yamaguchi K, Hayashizaki Y, Sekiya T
Oncogene Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1996 Aug 23;225(3):968-74. doi: 10.1006/bbrc.1996.1280.
In the human pancreatic cancer cell line PANC1, we detected several DNA fragments with abnormally intensified signals by restriction landmark genomic scanning. Major five of these fragments were cloned. All of the cloned fragments were mapped at the 19q13.1-13.2 region where the AKT2 oncogene was located. Southern blotting using the cloned DNA fragments and a fragment of AKT2 cDNA as probes revealed that the AKT2 gene was amplified in 3 of 12 pancreatic cancer cell lines analyzed including PANC1 and in 3 of 20 primary pancreatic cancers. The AKT2 gene was overexpressed in the 3 cell lines with the amplified gene. The results suggest that the AKT2 gene is a candidate oncogene activated by amplification in some human pancreatic cancers.
在人胰腺癌细胞系PANC1中，我们通过限制性内切酶标记基因组扫描检测到几个信号异常增强的DNA片段。克隆了其中主要的5个片段。所有克隆片段均定位于AKT2癌基因所在的19q13.1 - 13.2区域。以克隆的DNA片段和AKT2 cDNA片段为探针进行Southern印迹分析，结果显示在包括PANC1在内的12个分析的胰腺癌细胞系中的3个以及20个原发性胰腺癌中的3个中，AKT2基因发生了扩增。在具有扩增基因的3个细胞系中，AKT2基因过表达。这些结果表明，AKT2基因是某些人类胰腺癌中因扩增而激活的候选癌基因。
