Grabowski M, Johansson B B, Brundin P
Department of Neurology, University Hospital, Sweden.
Acta Neurochir Suppl. 1996;66:68-72. doi: 10.1007/978-3-7091-9465-2_12.
The aim was to study if neural grafts placed brain infarcts could improve functional recovery. The middle cerebral artery was occluded (MCAO) in 19 spontaneously hypertensive rats. Nine rats were sham operated. Twelve to 16 days after the ischemic insult, 9 of the MCAO rats received transplants of dissociated fetal neocortical tissue (MCAO-T) and 1, 3 and 6 months after transplantation surgery, the rats were behaviorally evaluated by a test for forelimb function. Infarct and transplant sizes were measured morphometrically. The remaining volume of the infarcted hemisphere was 66 +/- 7% (mean +/- SD) in the MCAO group and 71 +/- 9% in the MCAO-T group of the non-operated hemisphere. All grafted rats had surviving transplants. Contralateral to the lesion, paw-reaching was highly impaired in both infarcted groups compared with sham-operated controls with no significant difference between MCAO and MCAO-T. The lesion size correlated significantly with contralateral paw-reach performance at all test periods. We conclude that neocortical grafts did not alleviate the impaired forepaw function.
目的是研究植入脑梗死部位的神经移植物是否能改善功能恢复。对19只自发性高血压大鼠进行大脑中动脉闭塞（MCAO）手术。9只大鼠进行假手术。在缺血损伤后12至16天，9只MCAO大鼠接受了离体胎儿新皮质组织移植（MCAO-T），并在移植手术后1、3和6个月，通过前肢功能测试对大鼠进行行为评估。用形态测量法测量梗死灶和移植物的大小。在未手术的半球中，MCAO组梗死半球的剩余体积为66±7%（平均值±标准差），MCAO-T组为71±9%。所有移植大鼠的移植物均存活。与假手术对照组相比，两个梗死组损伤对侧的爪伸展功能均严重受损，MCAO组和MCAO-T组之间无显著差异。在所有测试阶段，梗死灶大小与对侧爪伸展性能显著相关。我们得出结论，新皮质移植物不能缓解前爪功能受损。
