Stowe R P, Pierson D L
KRUG Life Sciences, Lyndon B. Johnson Space Center, Houston, TX, USA.
Biotechniques. 1996 Jun;20(6):1088-9. doi: 10.2144/96206bc02.
This article describes a Microsoft Excel macro designed to calculate PCR master mix amounts based on variations in the DNA template amounts added to each tube, the total number of PCR assay tubes being set up or both. This macro uses a dynamic dialog box to quickly calculate and display the new component volumes after changes in one or both of the variables. The PCR assay mix protocol can then be printed in a format suitable for record keeping. This macro was designed for use with the Windows version of Excel but will also run on Macintosh computers.
本文介绍了一个Microsoft Excel宏，该宏旨在根据添加到每个试管中的DNA模板量的变化、要设置的PCR测定试管总数或两者来计算PCR主混合物的量。此宏使用动态对话框在一个或两个变量发生变化后快速计算并显示新的组分体积。然后可以以适合记录保存的格式打印PCR测定混合方案。此宏设计用于Excel的Windows版本，但也可以在Macintosh计算机上运行。
