Lähdetie J, Ajosenpää-Saari M, Mykkänen J
Department of Medical Genetics, University of Turku, Finland.
Environ Health Perspect. 1996 May;104 Suppl 3(Suppl 3):629-32. doi: 10.1289/ehp.104-1469614.
We have studied human spermatozoa from 24 normal, healthy unexposed men, 18 of whom were semen donors at the Sperm Bank in Turku, using multicolor fluorescence in situ hybridization with two chromosome-specific probes. The possible age-related increase in aneuploidy frequencies was assessed. Ten thousand spermatozoa were scored per individual for the presence of hyperploid, i.e., disomic and diploid, cells. The overall hybridization efficiency was 98.8%. The frequency of spermatozoa with two chromosome 1 signals was 11.5 +/- 5.2/10,000. The frequency of spermatozoa with two chromosome 7 signals was 6.4 +/- 3.9/10,000. Diploidy was present in 15.0 +/- 8.9/10,000 spermatozoa. Interindividual variation was quite large. No statistically significant correlation between age of the donors (range = 20-46 years) and the frequency of hyperploid spermatozoa was observed. The results give background information on the incidence of hyperploid spermatozoa in unexposed men and encourage the use of this novel technique of future studies on genetic effects in men exposed to potentially aneuploidogenic agents.
我们研究了24名正常、健康且未接触过有害物质的男性的精子，其中18人是图尔库精子库的精液捐赠者，采用了两种染色体特异性探针的多色荧光原位杂交技术。评估了非整倍体频率可能与年龄相关的增加情况。对每个个体的一万个精子进行评分，以确定超倍体细胞（即二体和二倍体细胞）的存在情况。总体杂交效率为98.8%。含有两个1号染色体信号的精子频率为11.5±5.2/10,000。含有两个7号染色体信号的精子频率为6.4±3.9/10,000。二倍体在15.0±8.9/10,000个精子中出现。个体间差异相当大。未观察到捐赠者年龄（范围为20 - 46岁）与超倍体精子频率之间存在统计学上的显著相关性。这些结果为未接触过有害物质的男性中超倍体精子的发生率提供了背景信息，并鼓励在未来对接触潜在非整倍体诱导剂的男性进行遗传效应研究时使用这种新技术。
