Härkönen K, Suominen J, Lähdetie J
Department of Medical Genetics, University of Turku, Kiinamyllynkatu 20, 20520 Turku, Finland.
Int J Androl. 2001 Aug;24(4):197-205. doi: 10.1046/j.1365-2605.2001.00280.x.
Recent studies have shown that aneuploidy in spermatozoa of infertile men with poor semen quality is increased. The purpose of this study was to determine whether poor sperm morphology is associated with the incidence of spermatozoa with numerical chromosome abnormalities. Semen samples from 20 infertile teratozoospermic men were studied using multicolour fluorescence in situ hybridization (FISH). Men were divided into four groups according to the proportion of normal sperm morphology: infertile men with <10% (group A, n=7), 10-19% (group B, n=6), and 20-29% (group C, n=7) of morphologically normal spermatozoa, and controls (group D, n=5) with > or =30% normal forms. Two hybridizations were performed. All the samples were analysed using probes for chromosomes 1 and 7 and, in addition, in group A and in controls with normal semen parameters probes for chromosomes X, Y and 18 were also used. Ten thousand spermatozoa were scored per hybridization. Severely teratozoospermic men (<10% normal forms) had significantly higher frequency of disomy 7, 18, YY, XY and diploidy in their spermatozoa when compared with controls. The results suggest that poor sperm morphology is associated with numerical chromosome abnormalities of spermatozoa. Severely teratozoospermic men may be at an increased risk of producing aneuploid offspring.
近期研究表明，精液质量差的不育男性精子中的非整倍体增多。本研究的目的是确定精子形态差是否与精子染色体数目异常的发生率相关。使用多色荧光原位杂交（FISH）技术对20名不育的畸形精子症男性的精液样本进行了研究。根据正常精子形态的比例将男性分为四组：正常精子形态比例<10%的不育男性（A组，n = 7）、10 - 19%的不育男性（B组，n = 6）、20 - 29%的不育男性（C组，n = 7），以及正常形态比例≥30%的对照组（D组，n = 5）。进行了两次杂交。所有样本均使用针对1号和7号染色体的探针进行分析，此外，A组和精液参数正常的对照组还使用了针对X、Y和18号染色体的探针。每次杂交对一万个精子进行评分。与对照组相比，严重畸形精子症男性（正常形态比例<10%）的精子中7、18号染色体双体、YY、XY和二倍体的频率显著更高。结果表明，精子形态差与精子染色体数目异常相关。严重畸形精子症男性产生非整倍体后代的风险可能增加。
