Yokomise H, Inui K, Wada H, Hitomi S
Department of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, Japan.
Surg Today. 1996;26(6):427-30. doi: 10.1007/BF00311930.
In our previous study, we demonstrated that high-dose 60Co irradiation was able to prevent rejection of canine tracheal allografts. To determine the maximum possible length of these grafts, in the present study we attempted to transplant five-ring and ten-ring tracheal allografts in two groups of five dogs each. Either five or ten rings were excised from donor tracheas and irradiated with 100,000 cGy of 60Co. The irradiated tracheal grafts were transplanted to replace either five- or ten-ring sections of the mediastinal tracheas removed from the recipient dogs. The grafts were covered with omental pedicles and no immunosuppressants were used. Graft incorporation was achieved in four of the five dogs in the five-ring groups, and three of these dogs survived for more than 700 days. However, four of the five animals in the ten-ring group died from tracheostenosis accompanied by ischemia within 3 weeks. These findings demonstrate the impossibility of performing ten-ring tracheal allotransplantation using irradiated grafts, even with omentopexy.
在我们之前的研究中，我们证明了高剂量60Co照射能够预防犬气管同种异体移植的排斥反应。为了确定这些移植物的最大可能长度，在本研究中，我们试图在两组各五只狗中移植五环和十环气管同种异体移植物。从供体气管中切除五或十个环，并用100,000 cGy的60Co进行照射。将照射后的气管移植物移植以替换从受体狗身上切除的纵隔气管的五或十环部分。移植物用网膜蒂覆盖，并且未使用免疫抑制剂。在五环组的五只狗中有四只实现了移植物整合，其中三只狗存活超过700天。然而，十环组的五只动物中有四只在3周内死于伴有缺血的气管狭窄。这些发现表明，即使进行网膜固定术，使用照射后的移植物进行十环气管同种异体移植也是不可能的。
