Yokomise H, Inui K, Wada H, Ueda M, Hitomi S
Division of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, Japan.
J Thorac Cardiovasc Surg. 1996 May;111(5):930-4. doi: 10.1016/s0022-5223(96)70366-5.
We previously reported that cryopreservation of tracheas for 1 month was possible in a canine tracheal autotransplantation model with use of a preservative solution containing trehalose. Realizing that the allogenicity of many organs is decreased by freezing, we examined the possibility of immunosuppressant-free canine tracheal allotransplantation after long-term cryopreservation. Six to 10 rings of the trachea were removed from donor dogs (n = 12), immersed in the preservative solution, and cryopreserved in a deep freezer at -85 degrees C for 285 +/- 28 days (cryopreservation group). Five rings of the mediastinal trachea of recipient dogs were removed. The cryopreserved tracheas were thawed and transplanted to replace the excised mediastinal tracheas. In a control group (n = 6), the graft was preserved in Euro-Collins solution at 10 degrees C for 16 to 17 hours. Allotransplantation of tracheas was done as in the cryopreservation group. The anastomotic site and graft were covered with an omental pedicle in both groups. In the cryopreservation group, every animal, except one that was killed for pathologic examination, survived more than 2 months. All the grafts of this group were viable, and no stenosis or tracheomalacia was observed. In the control group, most of the animals died within 1 month of tracheal stenosis caused by rejection. These findings reveal that immunosuppressant-free canine tracheal allotransplantation was consistently possible after long-term cryopreservation of the graft in a preservative solution containing trehalose. This simple method could solve both donor shortage and immunosuppression problems.
我们之前报道过，在犬类气管自体移植模型中，使用含有海藻糖的保存液可将气管冷冻保存1个月。鉴于冷冻可降低许多器官的同种异体抗原性，我们研究了长期冷冻保存后进行无免疫抑制剂犬类气管同种异体移植的可能性。从供体犬（n = 12）身上取下6至10个气管环，浸入保存液中，并在-85℃的低温冰箱中冷冻保存285±28天（冷冻保存组）。切除受体犬纵隔气管的5个环。将冷冻保存的气管解冻并移植以替代切除的纵隔气管。在对照组（n = 6）中，移植物在10℃的Euro-Collins溶液中保存16至17小时。气管同种异体移植的操作与冷冻保存组相同。两组的吻合部位和移植物均用带蒂大网膜覆盖。在冷冻保存组中，除1只用于病理检查而处死的动物外，每只动物均存活超过2个月。该组所有移植物均存活，未观察到狭窄或气管软化。在对照组中，大多数动物在1个月内死于排斥反应引起的气管狭窄。这些结果表明，在含有海藻糖的保存液中对移植物进行长期冷冻保存后，持续进行无免疫抑制剂的犬类气管同种异体移植是可行的。这种简单的方法可以解决供体短缺和免疫抑制问题。
