Dogasaki C, Nishijima M, Ohno N, Yadomae T, Miyazaki T
Faculty of Environmental Health Science, Azabu University, Kanagawa, Japan.
Biosci Biotechnol Biochem. 1996 Jul;60(7):1136-9. doi: 10.1271/bbb.60.1136.
Polyclonal antibodies against P-1, a pectic polysaccharide fraction extracted with 0.5 M NaOH from the kernels of Prunus mume and consisted of arabino-galacturonan, and I-3, the partial acid (0.1 M trifluoroacetic acid) hydrolysate of P-1, were prepared in Japanese white rabbits. Competitive ELISA experiments strongly suggested that anti P-1 and anti I-3 antibodies were different but P-1 and I-3 cross-reacted with each other to recognize a partly similar epitope structure. The reactivities of polysaccharide fractions from the raw flesh of P. mume, and the kernels of apricot and peach extracted with either water or sodium hydroxide were examined using both antisera by the indirect competitive ELISA method. The polysaccharide fractions extracted with sodium hydroxide solutions had the reactivities but not those extracted with cold and hot water. These facts suggested that the similar structure of polysaccharides to P-1 was present in the flesh of P. mume and the kernels of apricot and peach. However, neither pectin of apple nor citrus had reactivity with each antiserum. P-1 would be different in chemical structure from a commercially available pectin, a water-soluble polysaccharide from apple and citrus.
用0.5M氢氧化钠从青梅核中提取的果胶多糖级分P - 1，由阿拉伯半乳聚糖组成，以及P - 1的部分酸（0.1M三氟乙酸）水解产物I - 3，在日本白兔中制备了针对它们的多克隆抗体。竞争性ELISA实验强烈表明，抗P - 1和抗I - 3抗体不同，但P - 1和I - 3相互交叉反应以识别部分相似的表位结构。使用间接竞争ELISA方法，用两种抗血清检测了用水或氢氧化钠提取的青梅生果肉、杏仁核和桃仁中的多糖级分的反应性。用氢氧化钠溶液提取的多糖级分具有反应性，而用冷水和热水提取的则没有。这些事实表明，青梅果肉以及杏仁核和桃仁中存在与P - 1结构相似的多糖。然而，苹果和柑橘的果胶与每种抗血清均无反应性。P - 1在化学结构上与市售的果胶（一种来自苹果和柑橘的水溶性多糖）不同。
