Dogasaki C, Nishijima M, Ohno N, Yadomae T, Miyazaki T
Faculty of Environmental Health Science, Azabu University, Kanagawa, Japan.
Biosci Biotechnol Biochem. 1996 Nov;60(11):1831-6. doi: 10.1271/bbb.60.1831.
P-1 was partially hydrolyzed with 0.01, 0.05, and 0.1 M trifluoroacetic acid (TFA), successively, and the dialyzable (E-1, E-2, and E-3) and non-dialyzable (I-1, I-2, and I-3) fractions were prepared and analyzed chemically and immunochemically. Either I-1 or E-1 reacted with anti P-1 serum as strongly as P-1 and were mitogenic. The cross-reactivity of I-2 and I-3 was less than I-1 with anti P-1 serum. However, they were as mitogenic as I-1. The cross-reactivity of E-2 and E-3 to anti P-1 serum was also very weak, and they were not mitogenic. The E-1 fraction had a similar sugar composition to I-1 and P-1. E-2 was a monosaccharide, all of Ara, and would be from the linkage of furanosyl residues in P-1. The composition of E-3 was free from Ara and the structure of E-3 was similar to that of I-3. E-3 would be considered to be deleted arabinofuranose from E-1. These results suggest that the mitogenic activity measured by the alkaline phosphatase assay is a property of the core part, I-3, but that P-1 contains several epitopes other than the core part by the immunochemical analysis.
P - 1先后用0.01M、0.05M和0.1M的三氟乙酸（TFA）进行部分水解，制备出可透析部分（E - 1、E - 2和E - 3）和不可透析部分（I - 1、I - 2和I - 3），并进行化学和免疫化学分析。I - 1或E - 1与抗P - 1血清的反应强度与P - 1相同，且具有促有丝分裂活性。I - 2和I - 3与抗P - 1血清的交叉反应性低于I - 1。然而，它们的促有丝分裂活性与I - 1相同。E - 2和E - 3与抗P - 1血清的交叉反应性也非常弱，且无促有丝分裂活性。E - 1部分的糖组成与I - 1和P - 1相似。E - 2是一种单糖，全为阿拉伯糖，可能来自P - 1中呋喃糖残基的连接。E - 3的组成不含阿拉伯糖，其结构与I - 3相似。E - 3可认为是E - 1中阿拉伯呋喃糖缺失后的产物。这些结果表明，通过碱性磷酸酶测定法测得的促有丝分裂活性是核心部分I - 3的特性，但通过免疫化学分析表明P - 1除核心部分外还含有几个表位。
