Germaschewski V, Murray K
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland, UK.
J Virol Methods. 1996 Apr 26;58(1-2):21-32. doi: 10.1016/0166-0934(95)01980-4.
A random hexapeptide fusion-phage library was screened to isolate phage that bound antibodies in a serum induced by hepatitis B virus surface antigen (HBsAg). Analysis of the isolated phage and comparison of their displayed peptide sequences with the primary sequence of HBsAg revealed areas where three and four amino acid matches accumulated. Differential binding studies of individual phage clones with immune and pre-immune sera identified phage carrying sequences that matched with region 117-122 of HBsAg which may represent a linear epitope or part of a larger antigenic determinant. Synthetic hexapeptides representing this region competed for binding with the matching phage clones.
筛选了一个随机六肽融合噬菌体文库，以分离与乙肝病毒表面抗原（HBsAg）诱导的血清中的抗体结合的噬菌体。对分离出的噬菌体进行分析，并将其展示的肽序列与HBsAg的一级序列进行比较，发现了三个和四个氨基酸匹配积累的区域。对单个噬菌体克隆与免疫血清和免疫前血清进行差异结合研究，鉴定出携带与HBsAg 117-122区域匹配序列的噬菌体，该区域可能代表一个线性表位或更大抗原决定簇的一部分。代表该区域的合成六肽与匹配的噬菌体克隆竞争结合。
