Tan Wen Siang, Ho Kok Lian
Wen Siang Tan, Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
World J Gastroenterol. 2014 Sep 7;20(33):11650-70. doi: 10.3748/wjg.v20.i33.11650.
Hepatitis B virus (HBV) has killed countless lives in human history. The invention of HBV vaccines in the 20(th) century has reduced significantly the rate of the viral infection. However, currently there is no effective treatment for chronic HBV carriers. Newly emerging vaccine escape mutants and drug resistant strains have complicated the viral eradication program. The entire world is now facing a new threat of HBV and human immunodeficiency virus co-infection. Could phage display provide solutions to these life-threatening problems? This article reviews critically and comprehensively the innovative and potential applications of phage display in the development of vaccines, therapeutic agents, diagnostic reagents, as well as gene and drug delivery systems to combat HBV. The application of phage display in epitope mapping of HBV antigens is also discussed in detail. Although this review mainly focuses on HBV, the innovative applications of phage display could also be extended to other infectious diseases.
乙肝病毒（HBV）在人类历史上夺走了无数生命。20世纪乙肝疫苗的发明显著降低了病毒感染率。然而，目前对于慢性乙肝病毒携带者尚无有效的治疗方法。新出现的疫苗逃逸突变体和耐药菌株使病毒根除计划变得复杂。全世界目前正面临乙肝病毒与人类免疫缺陷病毒共同感染的新威胁。噬菌体展示能否为这些危及生命的问题提供解决方案？本文批判性地全面回顾了噬菌体展示在开发对抗乙肝病毒的疫苗、治疗剂、诊断试剂以及基因和药物递送系统方面的创新和潜在应用。还详细讨论了噬菌体展示在乙肝病毒抗原表位作图中的应用。尽管本综述主要聚焦于乙肝病毒，但噬菌体展示的创新应用也可扩展到其他传染病。
