Gut-Zangger P, Vretou E, Psarrou E, Pospischil A, Thoma R
Institut für Veterinärpathologie, Universität Zürich.
Schweiz Arch Tierheilkd. 1999;141(8):361-6.
466 sheep sera out of 19 flocks in Switzerland were examined by a competitive enzyme linked immunosorbent assay (cELISA) for antibodies against Chlamydia psittaci "serotype 1" ("ovine enzootic abortion"). Since numerous positive reactors were found in flocks without abortion history, 30 fecal samples out of two of these flocks were examined by PCR for evidence of chlamydial DNA. One of these samples turned out to contain DNA of Chlamydia psittaci "serotype 1". These results suggest, that in Switzerland "serotype 1" of Chlamydia psittaci is widespread not only as cause of chlamydial abortion but also as latent intestinal infection in sheep. The resulting difficulties for serological diagnosis of chlamydial abortion and possible solutions based on the cELISA are discussed. The complement fixation test (CFT), still considered as standard method for serological examination for Chlamydiae, has additionally been applied.
采用竞争酶联免疫吸附试验（cELISA）对瑞士19个羊群中的466份绵羊血清进行检测，以检测抗鹦鹉热衣原体“血清型1”（“绵羊地方流行性流产”）的抗体。由于在无流产病史的羊群中发现了大量阳性反应者，因此对其中两个羊群的30份粪便样本进行了PCR检测，以寻找衣原体DNA的证据。其中一个样本被证明含有鹦鹉热衣原体“血清型1”的DNA。这些结果表明，在瑞士，鹦鹉热衣原体“血清型1”不仅作为衣原体流产的病因广泛存在，而且作为绵羊的潜伏性肠道感染也广泛存在。本文讨论了衣原体流产血清学诊断中出现的困难以及基于cELISA的可能解决方案。此外，还应用了仍被视为衣原体血清学检测标准方法的补体结合试验（CFT）。
