Ikeda Y, Ozawa S, Ando N, Kitagawa Y, Ueda M, Kitajima M
Department of Surgery, Keio University School of Medicine, Tokyo, Japan.
Ann Thorac Surg. 1996 Sep;62(3):835-8. doi: 10.1016/s0003-4975(96)00392-x.
Accumulation of genetic abnormalities is linked to the development and progression of cancer. We therefore analyzed the correlation between the clinical characteristics of superficial esophageal squamous cell carcinoma patients and oncogene amplifications.
Between 1980 and 1991, there were 63 cases of superficial esophageal carcinoma (Tis and T1 cancer) at Keio University Hospital. The T1 cases were divided into two groups: T1a cases, in which the tumor had invaded the lamina propria, and T1b cases, in which the tumor had invaded the submucosa. DNA was isolated from paraffin-embedded blocks. Oncogene amplification was determined by slot-blot hybridization.
Amplification of int-2 and c-erbB was detected in 14 and 5, respectively, of the 54 cases. Three of 12 T1b patients with int-2 amplification died of distant organ metastasis. The survival rate for the group with int-2 amplification was significantly lower than that without int-2 amplification. All 4 T1b patients with c-erbB amplification had lymph node metastasis at operation.
These findings mean that genetic abnormalities are a useful marker for treating patients with superficial esophageal squamous cell carcinomas.
基因异常的积累与癌症的发生和发展相关。因此，我们分析了食管鳞状细胞癌浅表型患者的临床特征与癌基因扩增之间的相关性。
1980年至1991年间，庆应义塾大学医院有63例食管浅表癌（Tis和T1期癌）患者。T1期病例分为两组：肿瘤侵犯固有层的T1a期病例和肿瘤侵犯黏膜下层的T1b期病例。从石蜡包埋块中提取DNA。通过狭缝印迹杂交法检测癌基因扩增情况。
在54例病例中，分别检测到14例int-2基因和5例c-erbB基因扩增。12例int-2基因扩增的T1b期患者中有3例死于远处器官转移。int-2基因扩增组的生存率显著低于无int-2基因扩增组。所有4例c-erbB基因扩增的T1b期患者在手术时均有淋巴结转移。
这些发现表明，基因异常是治疗食管鳞状细胞癌浅表型患者的一个有用标志物。
