Booth R A, Weltman J Y, Yankov V I, Murray J, Davison T S, Rogol A D, Asplin C M, Johnson M L, Veldhuis J D, Evans W S
Department of Medicine, University of Virginia, Charlottesville 22908, USA.
J Clin Endocrinol Metab. 1996 Sep;81(9):3208-14. doi: 10.1210/jcem.81.9.8784071.
To test the hypothesis that FSH is secreted at least in part within discrete secretory bursts in women and that the characteristics of episodic FSH secretion are altered within differing gonadal hormone environments, we measured FSH by immunoradiometric assay every 10 min for 24 h in premenopausal women during the early follicular (EF), late follicular (LF), and midluteal (ML) phases of the menstrual cycle and in postmenopausal (PM) women (n = 8 in each group). Secretory events were evaluated using multiparameter deconvolution. FSH was secreted in an episodic manner, with the number of secretory bursts (per 24 h; mean +/- SEM) detected in LF (20 +/- 0.79) and PM (20 +/- 0.90) women being greater than that in EF (16 +/- 0.88) and ML (14 +/- 0.93) women. FSH secretory burst mass (milliinternational units per mL) was significantly higher in PM (12 +/- 1.6) than in EF (1.8 +/- 0.21), LF (3.1 +/- 1.3), or ML (0.8 +/- 0.11) women and primarily reflected a relative increase in the maximal secretory rate rather than increased burst half-duration. The estimated half-life (minutes) of endogenous FSH in LF women (155 +/- 18) was shorter than those calculated in EF (251 +/- 24), ML (277 +/- 38), and PM (231 +/- 18) women. Cross-correlation analysis showed strongly positive associations between successively paired serum FSH and LH concentrations in all four groups of women. Deconvolution of simultaneously obtained LH concentration-time series revealed statistically significant concordance (13-25%) between FSH and LH secretory episodes at a lag time of 0 min in EF, LF, and PM women and when LH secretory bursts led FSH secretory bursts by 10 min in ML phase women. However, as 75-87% of FSH and LH secretory pulses were discordant, we infer the operation of distinct control mechanisms in the generation of FSH and LH release episodes. In summary, these results suggest that FSH is secreted within discrete secretory bursts in women, that the mass and frequency of FSH secretory bursts differ in women exhibiting various gonadal hormone environments, and that FSH and LH secretory bursts occur coincidentally at a higher rate than expected on the basis of chance alone, but at such a low overall rate of concordance that distinct mechanisms probably operate to direct episodic FSH and LH secretory activity.
在女性中，促卵泡激素（FSH）至少部分是在离散的分泌脉冲中分泌的，并且在不同的性腺激素环境中，FSH的脉冲式分泌特征会发生改变。我们采用免疫放射分析法，在月经周期的卵泡早期（EF）、卵泡晚期（LF）和黄体中期（ML）对绝经前女性以及绝经后（PM）女性（每组n = 8）每10分钟测量一次FSH，持续24小时。使用多参数反卷积法评估分泌事件。FSH以脉冲方式分泌，LF期（20±0.79）和PM期（20±0.90）女性每24小时检测到的分泌脉冲数大于EF期（16±0.88）和ML期（14±0.93）女性。PM期女性的FSH分泌脉冲量（每毫升毫国际单位）显著高于EF期（1.8±0.21）、LF期（3.1±1.3）或ML期（0.8±0.11）女性，主要反映了最大分泌速率的相对增加，而非脉冲半持续时间的增加。LF期女性内源性FSH的估计半衰期（分钟）（155±18）短于EF期（251±24）、ML期（277±38）和PM期（231±18）女性计算得到的半衰期。交叉相关分析显示，在所有四组女性中，相继配对的血清FSH和LH浓度之间存在强正相关。对同时获得的LH浓度 - 时间序列进行反卷积分析发现，EF期、LF期和PM期女性在滞后时间为0分钟时，FSH和LH分泌事件之间存在统计学上显著的一致性（13 - 25%）；在ML期女性中，当LH分泌脉冲比FSH分泌脉冲提前10分钟时也存在一致性。然而，由于75 - 87% 的FSH和LH分泌脉冲不一致，我们推断在FSH和LH释放事件的产生过程中存在不同的控制机制。总之，这些结果表明，女性体内的FSH是在离散的分泌脉冲中分泌的，在不同性腺激素环境的女性中，FSH分泌脉冲的量和频率不同，并且FSH和LH分泌脉冲同时发生的频率高于仅基于偶然预期的频率，但总体一致性较低，可能存在不同机制来指导FSH和LH的脉冲式分泌活动。
