Department of Medicine, Health Sciences Center and the Biodynamics Institute, University of Virginia, Charlottesville, Virginia 22908, USA.
J Neuroendocrinol. 1990 Dec 1;2(6):845-52. doi: 10.1111/j.1365-2826.1990.tb00650.x.
Abstract To investigate the physiological regulation of luteinizing hormone (LH) secretory events and the endogenous clearance of this hormone, we applied multiple-parameter deconvolution analysis to serum LH concentration-time series obtained from normal women during three phases of the menstrual cycle. The number of significant LH secretory bursts (/24 h) was maximal in the late follicular (LF) phase (27 +/- 1.6; mean +/- SEM), minimal in the mid-luteal (ML) phase (10 +/-1.0) and intermediate in the early follicular (EF) phase (18 +/- 1.4). Similarly, the half-duration of the secretory impulse (min) was different at each phase of the cycle with values of 6.5+/-1.0, 3.5+/-0.9 and 11 +/- 1.1 during the EF, LF and ML phases, respectively. In contrast, there were no cycle-dependent differences in the LH half-life or in the total daily secretion of LH. When maximal secretory impulse amplitudes were examined, a putative bimodal distribution was found in the ML but not the EF or LF phases. The amplitudes for the large ML impulses, the LF and EF impulses and the small ML impulses were 0.95 +/- 0.05, 070 +/- 0.03, 0.43 +/- 0.02 and 0.26 +/- 0.02, respectively. The mass (mlU/ml) of hormone secreted within bursts was minimal in the LF phase (2.1 +/- 0.1), maximal in the large ML impulses (10.2 +/- 0.5) and intermediate in the EF (2.8 +/- 0.1) and small ML (3.1 +/-0.3) secretory impulses. There was no evidence of tonic (i.e. inter-secretory burst) LH secretion during any phase of the menstrual cycle. The early morning hours of the EF phase were characterized by fewer secretory bursts of greater amplitude. During the ML phase, autocorrelation analysis of inter-secretory burst intervals revealed a negative association indicating that high frequency events both precede and follow secretory pauses. In addition, secretory burst amplitude and both the preceding and following inter-secretory burst interval was correlated to secretory burst amplitude. These new data on the nature of regulated LH secretion indicate that specific facets of spontaneous LH secretory events are controlled throughout the menstrual cycle. Such observations offer a basis for defining altered secretory dynamics in a variety of pathophysiologic situations.
为了研究黄体生成素（LH）分泌事件的生理调节和这种激素的内源性清除，我们应用多参数反卷积分析，对正常女性在月经周期的三个阶段中获得的血清 LH 浓度时间序列进行了分析。在晚期卵泡期（LF），显著的 LH 分泌波数（/24 小时）最多（27+/-1.6；均值+/-SEM），在中期黄体期（ML）最少（10+/-1.0），在早期卵泡期（EF）居中（18+/-1.4）。同样，脉冲分泌的半持续时间（分钟）在每个周期阶段也不同，EF、LF 和 ML 期分别为 6.5+/-1.0、3.5+/-0.9 和 11+/-1.1。相反，LH 的半衰期或 LH 的日总分泌量没有周期依赖性差异。当检查最大脉冲分泌幅度时，在 ML 期发现了一种假定的双峰分布，但在 EF 或 LF 期没有。大 ML 脉冲、LF 和 EF 脉冲和小 ML 脉冲的幅度分别为 0.95+/-0.05、0.70+/-0.03、0.43+/-0.02 和 0.26+/-0.02。脉冲内分泌出的激素质量（mlU/ml）在 LF 期最小（2.1+/-0.1），在大 ML 脉冲时最大（10.2+/-0.5），在 EF（2.8+/-0.1）和小 ML（3.1+/-0.3）脉冲时居中。在月经周期的任何阶段都没有发现 LH 分泌的紧张（即脉冲间）。EF 期清晨，脉冲分泌波数较少，但幅度较大。在 ML 期，脉冲间间隔的自相关分析显示出负相关，表明高频事件既发生在分泌暂停之前，也发生在之后。此外，脉冲分泌幅度以及之前和之后的脉冲间间隔与脉冲分泌幅度相关。这些关于 LH 分泌调节的新数据表明，自发 LH 分泌事件的特定方面在整个月经周期中都受到控制。这些观察结果为定义各种病理生理情况下改变的分泌动力学提供了依据。
