Li H, Shen D T, O'Toole D, Davis W C, Knowles D P, Gorham J R, Crawford T B
Animal Diseases Research Unit, USDA Agricultural Research Service, Pullman, Washington 99164, USA.
Ann N Y Acad Sci. 1996 Jul 23;791:198-210. doi: 10.1111/j.1749-6632.1996.tb53526.x.
Malignant catarrhal fever (MCF) is a severe lymphoproliferative disease of certain domestic and wild ruminants. Two distinct but closely related viruses cause clinically indistinguishable syndromes in susceptible ruminant species: wildebeest-associated MCF virus (WA-MCFV) and sheep-associated MCF virus (SA-MCFV). Neither the pathogenesis nor the epidemiology of SA-MCF is understood, primarily because of a lack of adequate detection methods for the etiologic agent or antibody against that agent. Work designed to develop these tests has been under way in our laboratory. To obtain basic information about the virus, the in vitro growth properties of a US isolate of MCF virus were studied and its major viral proteins identified and characterized by a panel of monoclonal antibodies generated against the isolate. A monoclonal antibody to a broadly conserved epitope of MCF virus was identified, and a competitive-inhibition ELISA (CI-ELISA) was developed for detection of anti-MCF antibody in sheep and other ruminants. The monoclonal antibody (15-A) reacted with an epitope located on a glycoprotein complex, which was present in all isolates of MCF virus examined. Antibody from a wide variety of ruminants infected with MCF virus of both sheep and wildebeest origin competed with the monoclonal antibody 15-A for the epitope, which was not present on 14 other common ruminant viruses. The assay detected antibody in inapparently infected sheep, and in cattle, deer, and bison with clinical MCF. A PCR assay for DNA of the sheep-associated virus was developed, based on previously reported primers. Comparative studies demonstrated that the CI-ELISA was specific for MCFV antibody and that the PCR was more reliable for diagnosis of clinical MCF.
恶性卡他热（MCF）是某些家养和野生反刍动物的一种严重的淋巴细胞增生性疾病。两种不同但密切相关的病毒在易感反刍动物物种中引起临床上难以区分的综合征：牛羚相关的MCF病毒（WA-MCFV）和绵羊相关的MCF病毒（SA-MCFV）。SA-MCF的发病机制和流行病学都不清楚，主要是因为缺乏针对病原体或针对该病原体的抗体的适当检测方法。我们实验室一直在开展旨在开发这些检测方法的工作。为了获得有关该病毒的基本信息，研究了一株美国MCF病毒分离株的体外生长特性，并通过针对该分离株产生的一组单克隆抗体对其主要病毒蛋白进行了鉴定和表征。鉴定出一种针对MCF病毒广泛保守表位的单克隆抗体，并开发了一种竞争抑制ELISA（CI-ELISA）用于检测绵羊和其他反刍动物中的抗MCF抗体。单克隆抗体（15-A）与位于糖蛋白复合物上的一个表位发生反应，该表位存在于所有检测的MCF病毒分离株中。来自感染绵羊和牛羚来源的MCF病毒的各种反刍动物的抗体与单克隆抗体15-A竞争该表位，而该表位在其他14种常见反刍动物病毒上不存在。该检测方法在隐性感染的绵羊以及患有临床MCF的牛、鹿和野牛中检测到了抗体。基于先前报道的引物，开发了一种针对绵羊相关病毒DNA的PCR检测方法。比较研究表明，CI-ELISA对MCFV抗体具有特异性，而PCR对临床MCF的诊断更可靠。
