用于从痰标本中检测结核分枝杆菌的Amplicor和32千道尔顿PCR的比较。
Comparison of amplicor and 32-kilodalton PCR for detection of Mycobacterium tuberculosis from sputum specimens.
作者信息
Soini H, Agha S A, El-Fiky A, Viljanen M K
机构信息
Department in Turku, National Public Health Institute, Finland.
出版信息
J Clin Microbiol. 1996 Jul;34(7):1829-30. doi: 10.1128/JCM.34.7.1829-1830.1996.
Abstract
The commercial PCR test Amplicor was compared with the 32-kDa PCR for detection of Mycobacterium tuberculosis from 76 sputum specimens from Egyptian patients. Both tests performed with rather equal efficacy (resolved sensitivity of 88.9% for both tests; specificity of 98.0% for Amplicor and 93.9% for 32-kDa PCR). PCR was found to be useful in detection of auramine fluorescent stain-positive, culture-negative specimens.
摘要
将商业PCR检测试剂Amplicor与32-kDa PCR进行比较，以检测来自埃及患者的76份痰标本中的结核分枝杆菌。两种检测的效能相当（两种检测的分辨灵敏度均为88.9%；Amplicor的特异性为98.0%，32-kDa PCR的特异性为93.9%）。发现PCR在检测金胺荧光染色阳性、培养阴性的标本方面很有用。
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