Semenza G L, Rue E A, Iyer N V, Pang M G, Kearns W G
Center for Medical Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland, 21287, USA.
Genomics. 1996 Jun 15;34(3):437-9. doi: 10.1006/geno.1996.0311.
Hypoxia-inducible factor 1 (HIF-1) is a basic helix-loop-helix transcription factor that mediates homeostatic responses to hypoxia. HIF-1 is a heterodimer consisting of HIF-1alpha, which is encoded by the HIF1A gene, complexed with HIF-1beta, which is encoded by the ARNT gene. In this paper we report the assignment of Hif1a and HIF1A to mouse chromosome 12 and human chromosome 14, respectively. HIF1A was assigned to human chromosome 14q21-q24 by analysis of somatic cell hybrids and by fluorescence in situ hybridization. Hif1a was localized by interspecific backcross analysis within a region of mouse chromosome 12 encompassing >30 cM that demonstrates conservation of synteny with a region of human chromosome 14 extending from PAX9 at 14q12-q13 to IGHC at 14q32.33.
缺氧诱导因子1（HIF-1）是一种碱性螺旋-环-螺旋转录因子，介导对缺氧的稳态反应。HIF-1是一种异二聚体，由HIF-1α和HIF-1β组成，HIF-1α由HIF1A基因编码，HIF-1β由ARNT基因编码。在本文中，我们报告了Hif1a和HIF1A分别定位于小鼠12号染色体和人类14号染色体。通过体细胞杂种分析和荧光原位杂交，HIF1A被定位于人类14号染色体14q21-q24。通过种间回交分析，Hif1a被定位在小鼠12号染色体的一个区域内，该区域跨度超过30 cM，与人类14号染色体从14q12-q13的PAX9延伸至14q32.33的IGHC的区域显示出同线性保守性。
