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Amino acid analysis using derivatisation with 9-fluorenylmethyl chloroformate and reversed-phase high-performance liquid chromatography.

作者信息

Haynes P A, Sheumack D, Kibby J, Redmond J W

机构信息

School of Chemistry, Macquarie University, North Ryde, Australia.

出版信息

J Chromatogr. 1991 Mar 1;540(1-2):177-85. doi: 10.1016/s0021-9673(01)88807-5.

DOI:10.1016/s0021-9673(01)88807-5
PMID:8786243
Abstract

A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.

摘要

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