Lim K G, Wan H C, Bozza P T, Resnick M B, Wong D T, Cruikshank W W, Kornfeld H, Center D M, Weller P F
Department of Medicine, Beth Isreal Hospital, Harvard Medical School, Boston, MA 02215, USA.
J Immunol. 1996 Apr 1;156(7):2566-70.
Eosinophils and CD4+ lymphocytes are preferentially recruited into sites of allergic inflammation. A role for eosinophils in the recruitment of CD4+ lymphocytes has not been defined. We studied the capacity of human eosinophils to release chemoattractants for T lymphocytes. Supernatants of cultured eosinophils contained chemoattractant activity for lymphocytes, which was predominantly due to IL-16 (lymphocyte chemoattractant factor) and RANTES. With neutralizing Abs, eosinophil-derived lymphocyte chemotactic activity was diminished by a mean (+/- SEM) of 60 +/- 3% with polygonal anti-IL-16 Ab, 69 +/- 4% with anti-IL-16 mAb, 48 +/- 3% with anti-CD4 F(ab) (IL-16 receptor blockade), 40 +/- 4% with anti-RANTES mAb, and 88 +/- 5% with a combination of anti-IL-16 and anti-RANTES mAbs. IL-16 and RANTES were detectable in eosinophil-derived supernatants by ELISA. Eosinophils constitutively expressed mRNA transcripts for both IL-16 and RANTES detectable by reverse transcription-PCR and contained preformed IL-16 and RANTES demonstrable by ELISA of cell lysates and by immunocytochemistry of freshly isolated eosinophils. Thus, eosinophils are a source of two cytokines, IL-16 and RANTES, that are chemoattractants for lymphocytes as well as eosinophils. These data indicate that eosinophils could contribute cytokines to enhance the recruitment of additional populations of CD4+ lymphocytes and eosinophils.
嗜酸性粒细胞和CD4 +淋巴细胞优先被招募到过敏性炎症部位。嗜酸性粒细胞在CD4 +淋巴细胞招募中的作用尚未明确。我们研究了人类嗜酸性粒细胞释放T淋巴细胞趋化因子的能力。培养的嗜酸性粒细胞的上清液含有对淋巴细胞的趋化活性，这主要归因于IL-16（淋巴细胞趋化因子）和RANTES。使用中和抗体，嗜酸性粒细胞衍生的淋巴细胞趋化活性通过多克隆抗IL-16抗体平均（+/- SEM）降低60 +/- 3％，抗IL-16单克隆抗体降低69 +/- 4％，抗CD4 F（ab）（IL-16受体阻断）降低48 +/- 3％，抗RANTES单克隆抗体降低40 +/- 4％，抗IL-16和抗RANTES单克隆抗体组合降低88 +/- 5％。通过ELISA可在嗜酸性粒细胞衍生的上清液中检测到IL-16和RANTES。通过逆转录 - PCR可检测到嗜酸性粒细胞组成性表达IL-16和RANTES的mRNA转录本，并且通过细胞裂解物的ELISA和新鲜分离的嗜酸性粒细胞的免疫细胞化学可证明含有预先形成的IL-16和RANTES。因此，嗜酸性粒细胞是两种细胞因子IL-16和RANTES的来源，它们是淋巴细胞以及嗜酸性粒细胞的趋化因子。这些数据表明，嗜酸性粒细胞可以贡献细胞因子以增强额外的CD4 +淋巴细胞和嗜酸性粒细胞群体的招募。
