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通过在大肠杆菌中表达克隆粗糙脉孢菌乳清苷5'-磷酸羧化酶的结构基因。

Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.

作者信息

Buxton F P, Radford A

出版信息

Mol Gen Genet. 1983;190(3):403-5. doi: 10.1007/BF00331067.

DOI:10.1007/BF00331067
PMID:6308396
Abstract

A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'-monophosphate carboxylase in E. coli is within the Neurospora sequence.

摘要

利用质粒pRK9中的粗糙脉孢菌基因文库对大肠杆菌中的嘧啶营养缺陷型进行互补。获得了两个可互补大肠杆菌pyrF突变体的质粒。这些质粒与粗糙脉孢菌DNA杂交，并转化粗糙脉孢菌的pyr-4菌株。在大肠杆菌中表达乳清苷5'-单磷酸羧化酶时所使用的启动子位于粗糙脉孢菌序列内。

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1
Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.通过在大肠杆菌中表达克隆粗糙脉孢菌乳清苷5'-磷酸羧化酶的结构基因。
Mol Gen Genet. 1983;190(3):403-5. doi: 10.1007/BF00331067.
2
Sequence analysis of the pyr-4 (orotidine 5'-P decarboxylase) gene of Neurospora crassa.粗糙脉孢菌的pyr-4（乳清苷5'-磷酸脱羧酶）基因的序列分析。
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Cloning and physical analysis of the pyrF gene (coding for orotidine-5'-phosphate decarboxylase) from Escherichia coli K-12.来自大肠杆菌K-12的pyrF基因（编码乳清苷-5'-磷酸脱羧酶）的克隆及物理分析。
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Cloning of the afl-2 gene involved in aflatoxin biosynthesis from Aspergillus flavus.从黄曲霉中克隆参与黄曲霉毒素生物合成的afl-2基因。
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