Perkins S L, Adams G A
Division of Biochemistry, Ottawa Civic Hospital, Canada.
Clin Chem. 1996 Sep;42(9):1467-73.
We evaluated the Du Pont Axial Separation Technology (AST) system, which permanently separates a blood specimen into serum or plasma and cells in 1 min. Blood was collected in axial processing containers by standard venipuncture techniques and processed in an Axial Separation Module. Paired patient samples (n = 48 serum pairs, n = 50 plasma pairs) revealed no clinically significant differences in results for common chemistry analytes from samples collected in Becton Dickinson Vacutainer Tubes and centrifuged conventionally vs those from samples collected and processed in the AST system, except for a bias in serum (but not plasma) lactate dehydrogenase activity. This bias was dependent on the extent of stretching of the clot during processing and could be minimized by clotting the blood sample horizontally (axially). We believe the rapid separation of blood by the AST system has the potential to substantially reduce preanalytical specimen handling time.
我们评估了杜邦轴向分离技术（AST）系统，该系统可在1分钟内将血液标本永久性地分离为血清或血浆及细胞。通过标准静脉穿刺技术将血液采集到轴向处理容器中，并在轴向分离模块中进行处理。配对的患者样本（48对血清样本，50对血浆样本）显示，与在Becton Dickinson真空采血管中采集并常规离心的样本相比，在AST系统中采集和处理的样本，其常见化学分析物的结果在临床上无显著差异，但血清（而非血浆）乳酸脱氢酶活性存在偏差。这种偏差取决于处理过程中凝块的拉伸程度，通过水平（轴向）凝固血样可将其降至最低。我们认为，AST系统对血液的快速分离有潜力大幅减少分析前标本处理时间。
