Ullman E F, Kirakossian H, Switchenko A C, Ishkanian J, Ericson M, Wartchow C A, Pirio M, Pease J, Irvin B R, Singh S, Singh R, Patel R, Dafforn A, Davalian D, Skold C, Kurn N, Wagner D B
Research Department, Behring Diagnostics Inc., San Jose, CA 95161-9013,
Clin Chem. 1996 Sep;42(9):1518-26.
Luminescent oxygen channeling assay (LOCI) is a homogeneous immunoassay method capable of rapid, quantitative determination of a wide range of analytes--including high and very low concentrations of large and small molecules, free (unbound) drugs, DNA, and specific IgM. Assays have been carried out in serum and in lysed blood. Reliable detection of 1.25 microU/L thyrotropin (TSH) and 5 ng/L hepatitis B surface antigen (HBsAg) corresponds to detection limits approximately 3- and approximately 20-fold lower, respectively, than those of the best commercially available assays. An assay of chorionic gonadotropin is capable of quantification over a 10(6)-fold range of concentrations without a biphasic response. Latex particle pairs are formed in the assay through specific binding interactions by sequentially combining the sample and two reagents. One particle contains a photosensitizer, the other a chemiluminescer. Irradiation causes photosensitized formation of singlet oxygen, which migrates to a bound particle and activates the chemiluminescer, thereby initiating a delayed luminescence emission. Assay times range from 1 to 25 min.
发光氧通道分析（LOCI）是一种均相免疫分析方法，能够快速、定量地测定多种分析物，包括高浓度和极低浓度的大分子和小分子、游离（未结合）药物、DNA以及特异性IgM。该分析已在血清和溶血血液中进行。可靠检测1.25微单位/升促甲状腺激素（TSH）和5纳克/升乙型肝炎表面抗原（HBsAg），其检测限分别比市售最佳分析方法低约3倍和约20倍。绒毛膜促性腺激素分析能够在10^6倍的浓度范围内进行定量，且无双相响应。在分析中，通过依次将样品与两种试剂结合，通过特异性结合相互作用形成乳胶颗粒对。一个颗粒含有光敏剂，另一个含有化学发光剂。照射会导致单线态氧的光敏形成，单线态氧迁移到结合颗粒并激活化学发光剂，从而引发延迟发光发射。分析时间为1至25分钟。
