Watanabe T, Okada A, Mikami T, Matsumoto T, Suzuki M
Department of Microbiology, Tohoku College of Pharmacy, Sendai, Japan.
Biol Pharm Bull. 1995 Dec;18(12):1643-6. doi: 10.1248/bpb.18.1643.
The number of spleen Mac-1-positive cells was markedly increased in methylcholanthrene-induced Meth-A fibrosarcoma (Meth-A)-implanted mice. When the sera of Meth-A-implanted mice were added to normal bone marrow cells, granulocyte and macrophage colony stimulating factor (GM-CSF)-dependent growth of bone marrow cells was significantly enhanced. Analysis of the Meth-A-implanted mice sera showed that the levels of serum transferrin were markedly elevated. When the sera of Meth-A-implanted mice were pretreated with anti-mouse transferrin antibody, the enhancement of GM-CSF-dependent cell growth was almost abolished. Cell growth was also stimulated by the addition of transferrin which also caused an increase in the number of Mac-1-positive cells. Analysis of the effect of transferrin on bone marrow cells showed that the response of the cells to GM-CSF was significantly increased by preincubation with transferrin.
在植入甲基胆蒽诱导的Meth-A纤维肉瘤（Meth-A）的小鼠中，脾脏Mac-1阳性细胞的数量显著增加。当将植入Meth-A的小鼠血清添加到正常骨髓细胞中时，骨髓细胞依赖粒细胞和巨噬细胞集落刺激因子（GM-CSF）的生长显著增强。对植入Meth-A的小鼠血清分析表明，血清转铁蛋白水平显著升高。当用抗小鼠转铁蛋白抗体预处理植入Meth-A的小鼠血清时，GM-CSF依赖的细胞生长增强几乎被消除。添加转铁蛋白也刺激细胞生长，这也导致Mac-1阳性细胞数量增加。转铁蛋白对骨髓细胞作用的分析表明，转铁蛋白预孵育可显著增加细胞对GM-CSF的反应。
