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用于分析鼻病毒蛋白酶抑制剂的简单体外翻译试验。

Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases.

作者信息

Heinz B A, Tang J, Labus J M, Chadwell F W, Kaldor S W, Hammond M

机构信息

Infectious Disease Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.

出版信息

Antimicrob Agents Chemother. 1996 Jan;40(1):267-70. doi: 10.1128/AAC.40.1.267.

DOI:10.1128/AAC.40.1.267
PMID:8787922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC163099/
Abstract

We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.

摘要

我们开发了一种简单的体外翻译方法，用于分析在肽底物测定中抑制鼻病毒3C蛋白酶但无抗病毒活性的化合物。这种补充测定法能提供定性和定量结果，可检测天然多蛋白形式的3CD蛋白酶的抑制情况。

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本文引用的文献

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Purification of recombinant human rhinovirus 14 3C protease expressed in Escherichia coli.在大肠杆菌中表达的重组人鼻病毒14 3C蛋白酶的纯化
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Expression of virus-encoded proteinases: functional and structural similarities with cellular enzymes.病毒编码蛋白酶的表达：与细胞酶的功能和结构相似性
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Picornavirus inhibitors.小核糖核酸病毒抑制剂
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Fine-needle aspiration biopsy of the breast.乳房细针穿刺活检
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In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.体外分子遗传学作为一种确定脊髓灰质炎病毒3C蛋白酶差异切割特异性的工具。
Nucleic Acids Res. 1987 Mar 11;15(5):2069-88. doi: 10.1093/nar/15.5.2069.
10
Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase.用纯化的T7 RNA聚合酶合成传染性脊髓灰质炎病毒RNA
Proc Natl Acad Sci U S A. 1986 Apr;83(8):2330-4. doi: 10.1073/pnas.83.8.2330.