用于分析鼻病毒蛋白酶抑制剂的简单体外翻译试验。
Simple in vitro translation assay to analyze inhibitors of rhinovirus proteases.
作者信息
Heinz B A, Tang J, Labus J M, Chadwell F W, Kaldor S W, Hammond M
机构信息
Infectious Disease Research, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.
出版信息
Antimicrob Agents Chemother. 1996 Jan;40(1):267-70. doi: 10.1128/AAC.40.1.267.
Abstract
We have developed a simple in vitro translation method to analyze compounds that inhibit the rhinovirus 3C protease in peptide substrate assays but demonstrate no antiviral activity. This complementary assay, which provides both qualitative and quantitative results, detects the inhibition of the 3CD protease in the native polyprotein form.
摘要
我们开发了一种简单的体外翻译方法，用于分析在肽底物测定中抑制鼻病毒3C蛋白酶但无抗病毒活性的化合物。这种补充测定法能提供定性和定量结果，可检测天然多蛋白形式的3CD蛋白酶的抑制情况。
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