Araki M, Fukumatsu Y, Katabuchi H, Shultz L D, Takahashi K, Okamura H
Second Department of Pathology, Kumamoto University School of Medicine, Japan.
Biol Reprod. 1996 Feb;54(2):478-84. doi: 10.1095/biolreprod54.2.478.
To clarify the role of macrophages and macrophage colony-stimulating factor (M-CSF) in follicular development and ovulation, the processes of folliculogenesis and ovulation, numerical changes in macrophages, and proliferative capacity of granulosa cells were examined in op/op mice before or after daily M-CSF administration. The natural estrous cycle was determined daily by means of vaginal smears. The number of ovulated ova in both fallopian tubes was significantly smaller in op/op mice than in normal littermates. Such ova markedly increased in number after daily M-CSF administration. The numbers of both antral and mature follicles in the proestrous ovary were markedly lower in op/op mice than in the controls and increased after daily M-CSF administration. Flash-labeling with [3H]thymidine showed that the proliferative capacity of granulosa cells in antral follicles was reduced in op/op mice but elevated after daily M-CSF administration. Numbers of granulosa cells and macrophages in the antral follicles were significantly decreased in op/op mice but were increased after M-CSF treatment. All these data provide evidence that macrophages are implicated in the process of folliculogenesis and ovulation.
为阐明巨噬细胞和巨噬细胞集落刺激因子（M-CSF）在卵泡发育和排卵中的作用，在每日给予M-CSF之前或之后，对op/op小鼠的卵泡发生和排卵过程、巨噬细胞数量变化以及颗粒细胞的增殖能力进行了研究。通过阴道涂片每日确定自然发情周期。op/op小鼠双侧输卵管中排卵的卵子数量明显少于正常同窝小鼠。每日给予M-CSF后，此类卵子数量显著增加。动情前期卵巢中窦状卵泡和成熟卵泡的数量在op/op小鼠中均明显低于对照组，每日给予M-CSF后增加。用[3H]胸腺嘧啶进行脉冲标记显示，op/op小鼠窦状卵泡中颗粒细胞的增殖能力降低，但每日给予M-CSF后升高。op/op小鼠窦状卵泡中颗粒细胞和巨噬细胞的数量显著减少，但M-CSF治疗后增加。所有这些数据都证明巨噬细胞参与了卵泡发生和排卵过程。
