Zahm D S, Williams E, Wohltmann C
Department of Anatomy and Neurobiology, St. Louis University School of Medicine, Missouri 63104, USA.
J Comp Neurol. 1996 Jan 8;364(2):340-62. doi: 10.1002/(SICI)1096-9861(19960108)364:2<340::AID-CNE11>3.0.CO;2-T.
Retrograde and anterograde tract-tracing studies were carried out to determine whether the capacity of the nucleus accumbens to influence the thalamic mediodorsal nucleus via ventral striatopallidothalamic connections disproportionately favors the shell over the core subterritory. After injections of Fluoro-Gold into the mediodorsal thalamic nucleus, retrogradely labeled neurons were detected in sections also processed for calbindin-D 28-kD and neurotensin immunoreactivities to facilitate identification of subterritories in the ventral pallidum. Fluoro-Gold-labeled cells were counted in series of sections cut through the ventral pallidum, rostral globus pallidus, nucleus of the vertical limb of the diagonal band, preoptic region, lateral hypothalamus, and the sublenticular gray region, including parts of the extended amygdala. Data were expressed as cells/unit area and as percentages of all labeled forebrain cells. Mediodorsal nucleus-projecting rostroventral forebrain neurons were most numerous in the ventromedial part of the subcommissural ventral pallidum and pallidal parts of the olfactory tubercle. Few were observed in the dorsolateral part of the subcommissural ventral pallidum. In addition, following injections into the ventral pallidum, anterogradely transported biotinylated dextran amine was evaluated in sections processed for calbindin or tyrosine hydroxylase immunoreactivities. Injection into the ventromedial part of the subcommissural ventral pallidum resulted in robust anterograde labeling of the medial segment of the mediodorsal nucleus and ventral tegmental area and weak labeling of the substantia nigra and subthalamic nucleus. Conversely, after injection into the dorsolateral part of the subcommissural ventral pallidum, anterograde labeling was weak in the mediodorsal nucleus and ventral tegmental area, but robust in the substantia nigra and subthalamic nucleus. The results are consistent with a predominant accumbens shell influence on the mediodorsal nucleus and with cortico-ventral striatopallidal-thalamocortical pathways that begin and end in different parts of the frontal lobe.
进行了逆行和顺行示踪研究，以确定伏隔核通过腹侧纹状体苍白球丘脑连接影响丘脑背内侧核的能力是否对壳区的偏爱程度超过核心亚区。在将荧光金注入丘脑背内侧核后，在同时进行钙结合蛋白-D 28-kD和神经降压素免疫反应处理的切片中检测到逆行标记的神经元，以方便识别腹侧苍白球中的亚区。在穿过腹侧苍白球、嘴侧苍白球、斜角带垂直部核、视前区、外侧下丘脑以及包括部分扩展杏仁核的豆状核下灰质区域的一系列切片中，对荧光金标记的细胞进行计数。数据以细胞/单位面积以及所有标记的前脑细胞的百分比表示。投射到背内侧核的嘴侧腹侧前脑神经元在连合下腹侧苍白球的腹内侧部分和嗅结节的苍白球部分数量最多。在连合下腹侧苍白球的背外侧部分观察到的较少。此外，在将药物注入腹侧苍白球后，在进行钙结合蛋白或酪氨酸羟化酶免疫反应处理的切片中评估顺行运输的生物素化葡聚糖胺。注入连合下腹侧苍白球的腹内侧部分导致背内侧核内侧段和腹侧被盖区出现强烈的顺行标记，而黑质和丘脑底核的标记较弱。相反，在注入连合下腹侧苍白球的背外侧部分后，背内侧核和腹侧被盖区的顺行标记较弱，但黑质和丘脑底核的标记较强。这些结果与伏隔核壳区对背内侧核的主要影响以及始于和止于额叶不同部位的皮质-腹侧纹状体苍白球丘脑皮质通路一致。
