Belay T, Cherniak R, O'Neill E B, Kozel T R
Department of Chemistry, Georgia State University, Atlanta 30303, USA.
J Clin Microbiol. 1996 Feb;34(2):466-70. doi: 10.1128/jcm.34.2.466-470.1996.
A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.
描述了一种基于直接分析培养上清液中主要的型特异性荚膜抗原——葡糖醛酸木糖甘露聚糖,对新型隐球菌进行血清分型的方法。通过吸收多克隆兔抗血清(日本东京医创株式会社)或选定的抗新型隐球菌单克隆抗体制备的因子血清,用于斑点酶测定以检测抗原的存在。