通过斑点酶法对新型隐球菌进行血清分型。
Serotyping of Cryptococcus neoformans by dot enzyme assay.
作者信息
Belay T, Cherniak R, O'Neill E B, Kozel T R
机构信息
Department of Chemistry, Georgia State University, Atlanta 30303, USA.
出版信息
J Clin Microbiol. 1996 Feb;34(2):466-70. doi: 10.1128/jcm.34.2.466-470.1996.
Abstract
A method is described for the serotyping of Cryptococcus neoformans based on direct analysis of culture supernatants for the major type-specific capsular antigen, glucuronoxylomannan. Factor sera prepared by absorption of polyclonal rabbit antisera (Iatron Laboratories, Inc., Tokyo, Japan) or selected anti-C. neoformans monoclonal antibodies were used in a dot enzyme assay to detect the presence of antigen.
摘要
描述了一种基于直接分析培养上清液中主要的型特异性荚膜抗原——葡糖醛酸木糖甘露聚糖，对新型隐球菌进行血清分型的方法。通过吸收多克隆兔抗血清（日本东京医创株式会社）或选定的抗新型隐球菌单克隆抗体制备的因子血清，用于斑点酶测定以检测抗原的存在。
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本文引用的文献
1
2
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J Immunol. 1965 Jun;94:916-20.
3
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Carbohydr Res. 1995 Oct 23;276(2):365-86. doi: 10.1016/0008-6215(95)00167-r.
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