Li N, Li K A, Tong S Y
Department of Chemistry, Peking University, Beijing, People's Republic of China.
Anal Biochem. 1996 Jan 15;233(2):151-5. doi: 10.1006/abio.1996.0022.
A simultaneous determination of micro amounts of albumin and globulin fractions by fluorometry with only reagent alpha,beta,gamma,delta-tetra(4'-carboxyphenyl)porphin (TCPP) in the aqueous solution has been developed. Under the experimental conditions (5.6 x 10(-7) mol/liter of TCPP, pH 3.0, and at room temperature), the linear range of this assay was 0.09-12 microgram/ml and the detection limits (3 S/N) were 60 ng/ml for albumin and 90 ng/ml for globulin, respectively. The human serum samples were measured satisfactorily by using this method.
已开发出一种仅使用试剂α,β,γ,δ-四(4'-羧基苯基)卟啉(TCPP)在水溶液中通过荧光法同时测定微量白蛋白和球蛋白组分的方法。在实验条件下(5.6×10⁻⁷摩尔/升的TCPP、pH 3.0和室温)，该测定方法的线性范围为0.09 - 12微克/毫升，白蛋白和球蛋白的检测限(3S/N)分别为60纳克/毫升和90纳克/毫升。使用该方法对人血清样品进行了令人满意的测定。
