Kuraya N, Hase S
Department of Chemistry, Osaka University College of Science, Japan.
Anal Biochem. 1996 Jan 15;233(2):205-11. doi: 10.1006/abio.1996.0029.
Two-dimensional HPLC mapping of O-linked sugar chains was studied. Twenty-seven pyridylamino (PA-) derivatives of O-linked sugar chains liberated from glycoproteins by hydrazinolysis-N-acetylation were separated by reversed-phase and size-fractionation HPLC. Combining the elution positions obtained by these two HPLC techniques allows the construction of a two-dimensional sugar map which can be used to analyze the structure of PA-O-linked sugar chains. As in the case of PA-N-linked sugar chains, the additivity rule which correlates chemical structures with elution positions was also found in the case of PA-O-linked sugar chains. This separation system was effectively applied to the elucidation of minute amounts of PA-O-linked oligosaccharides.
对O-连接糖链的二维高效液相色谱图谱进行了研究。通过肼解-N-乙酰化从糖蛋白中释放出的27种O-连接糖链的吡啶氨基（PA-）衍生物，通过反相和尺寸排阻高效液相色谱进行分离。将这两种高效液相色谱技术获得的洗脱位置相结合，可构建二维糖图谱，用于分析PA-O-连接糖链的结构。与PA-N-连接糖链的情况一样，在PA-O-连接糖链的情况下也发现了将化学结构与洗脱位置相关联的加和规则。该分离系统有效地应用于微量PA-O-连接寡糖的结构解析。
